肝门静脉感染的方式建立肝泡状棘球蚴小鼠模型的可行性研究  

Feasibility study and analysis of establishing a mouse model of hepatic alveolar echinococcosis by hepatic portal vein infection

作　　者：田蔚莉 季文超 李军 刘文亚 何良浩 TIAN Weili;JI Wenchao;LI Jun;LIU Wenya;HE Lianghao(Imaging Center,Affiliated Tumour Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830000,China;Imaging Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi,Xinjiang 830054,China)

机构地区：[1]新疆医科大学附属肿瘤医院影像中心,新疆乌鲁木齐830000 [2]新疆医科大学第一附属医院影像中心,新疆乌鲁木齐830054

出　　处：《热带医学杂志》2024年第5期662-665,F0004,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金面上项目(81772008)。

摘　　要：目的探讨经肝门静脉感染的方式建立肝泡状棘球蚴小鼠模型的可行性,为临床实验研究提供良好的建模方法及依据。方法本实验选用20只C57小鼠建立肝泡状棘球蚴模型。首先无菌处死腹部已经感染泡状棘球蚴的长爪沙鼠,将沙鼠腹腔剖开,取出泡球蚴组织,制备成浓度为20%的混悬液(20000个原头节/mL),然后经肝门静脉注射200~300μL混悬液到C57小鼠体内,实验过程中小鼠死于手术原因2只,死于麻醉原因2只,死亡率为20%;剩余16只小鼠中,4只未感染成功,作为未感染组,12只感染成功的小鼠作为感染组,感染建模成功率为75%。术后正常喂养小鼠10周,最后采用腹部超声成像、核磁共振成像及组织病理学观察小鼠肝脏病变情况。结果未感染组小鼠肝脏大小形态及信号未见异常;感染组超声显示肝左叶可见明确病灶,病灶边界清晰,回声不均匀,可见高低混杂回声,内可见血流;核磁显示感染组肝左外叶可见明确病灶,边界清晰,病灶以稍长T2信号为主,并可见多发小囊泡影。未感染组病理结果为正常肝脏;感染组肝脏病灶内可观察到囊状泡以及纤维结构组织,并且囊泡周围的纤维成分较多,周边可以见到炎性反应带,内含炎性细胞、微血管及纤维组织。结论经肝门静脉感染的方式,可以建立肝泡状棘球蚴模型,该方法简单易行,并且成功率相对较高。Objective Discusses the feasibility of establishing a mouse model of hepatic alveolar echinococcosis through the hepatic portal vein,provide a good modeling method and basis for clinical experimental research. Methods In this experiment,twenty C57 mice were used to establish a model of hepatic alveolar echinococcosis. First, the long-clawed gerbils whose abdomens have been infected with alveolar Echinococcus were aseptically killed. The abdominal cavity of the gerbil was cut open, the cystic echinococcal tissue was removed, and a suspension with a concentration of 20% was prepared(20 000 protoscolex cells/mL). The 200-300 μL suspension was injected into C57 mice via the hepatic portal vein. During the experiment, 2 mice died of surgery and 2 of causes of anesthesia, with a mortality rate of 20%. Of the remaining 16mice, 4 were not successfully infected,uesd as non-infected group,12 mice successfully infected were used as the infection group,the infection modeling success rate was 75%. The mice were fed normally for 10 weeks after the operation. Finally, abdominal ultrasound imaging,nuclear magnetic resonance imaging, and histopathology were used to observe the liver lesions of the mice. Results The results showed that there were no abnormalities in the size, morphology, and signal of the liver in the non-infected group mice. The infection group ultrasound showed clear lesions in the left lobe of the liver, with clear boundaries and uneven echoes, mixed high and low echoes, and blood flow visible inside. Magnetic resonance imaging showed clear lesions in the left outer lobe of the liver in the infection group, with clear boundaries. The lesions were mainly characterized by slightly longer T2 signals, and multiple small cystic shadows were visible. The pathological results of the noninfected group were normal liver. Cystic vesicles and fibrous tissue can be observed in the liver lesions of the infection group,and there are many fibrous components around the vesicles. Inflammatory reaction zones can be seen around

关 键 词：肝泡状棘球蚴 肝门静脉 核磁共振成像 

分 类 号：R532.32[医药卫生—内科学]