张氏肌痹洗方熏洗治疗膝骨关节炎的机制研究  

Study on Mechanism of Fumigation with Zhang's Muscular Arthralgia Washing Prescription in Treating Knee Osteoarthritis

作　　者：何晓丹 鲍荣华 丁芳平 杨信波 HE Xiaodan;BAO Ronghua;DING Fangping;YANG Xingbo(Hangzhou Fuyang Bone Injury Hospital of Traditional Chinese Medicine,Hangzhou 311400,China)

机构地区：[1]杭州市富阳中医骨伤医院,杭州311400

出　　处：《中国中医骨伤科杂志》2024年第6期30-34,共5页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:基于Piezo机械敏感性离子通道探究张氏肌痹洗方熏洗治疗膝骨关节炎(KOA)的机制。方法:选取成年雌性SD大鼠,随机分为空白组、模型组、模型+阻断剂组、模型+中药熏洗组和模型+中药熏洗+阻断剂组,每组各10只。其中模型组的大鼠采用下肢石膏固定法构建膝骨关节炎模型;模型+阻断剂组于造模后第1天开始,分别在右侧足底局部注射GsMTx-4,100μL/次,1次/d,连续7 d;模型+中药熏洗组和模型+中药熏洗+阻断剂组在造模后第4天分别介入中药熏洗,中药熏洗20 min, 1次/d,连续干预21 d。之后各组大鼠处死,截取左后肢膝关节滑膜组织作为标本,采用ELISA法测定关节液中IL-1β、IL-6和TNF-α的表达量;Western Blot法检测大鼠左膝关节滑膜组织中Piezo两型及ERK1/2的蛋白表达量;RT-PCR法检测Piezo两型及ERK1/2的mRNA相对表达量。结果:与空白组比较,模型组大鼠膝关节滑膜组织中Piezo两型及ERK1/2的表达量均明显升高,差异有统计学意义(P<0.05);与模型组比较,模型+阻断剂组大鼠左膝关节滑膜组织中Piezo两型及ERK1/2的含量均明显降低,差异有统计学意义(P<0.05)。与空白组比较,模型组大鼠膝关节腔内关节液中IL-1β、IL-6和TNF-α表达水平均明显升高,差异有统计学意义(P<0.05);与模型组比较,模型+中药熏洗组和模型+中药熏洗+阻断剂组大鼠膝关节腔内关节液中IL-1β、IL-6和TNF-α表达水平均明显降低,差异有统计学意义(P<0.05)。与空白组比较,模型组大鼠左膝关节滑膜组织中Piezo两型、ERK1/2表达水平及mRNA表达量均明显升高,差异有统计学意义(P<0.05);与模型组比较,模型+中药熏洗组和模型+中药熏洗+阻断剂组大鼠左膝关节滑膜组织中Piezo两型、ERK1/2表达水平及mRNA表达量均明显降低,差异有统计学意义(P<0.05)。结论:张氏肌痹洗方熏洗能够通过调控Piezo机械敏感性离子通道改善炎症因子水平,进而缓解关节的退变Objective:To explore the mechanism of fumigation with Zhang's muscular arthralgia washing prescription in treating knee osteoarthritis(KOA)based on Piezo mechanical sensitivity ion channel.Methods:Adult female SD rats were selected and randomly divided into blank group,model group,model+blocker group,model+TCM washing group,model+TCM washing+blocker group,respectively,with 10 animals in each group.The rats in the model group constructed KOA model by lower limb plaster.The model+blocker group injected GsMTx-4 on the right foot on the first day,100 μL,once a day for 7 consecutive days.The model+TCM washing group and model+TCM washing+blocker group were respectively intervened with TCM washing on the 4th day after modeling,for 20 min each time,once a day,for 21 consecutive days.Rats were killed later and synovial tissue of left hindlimb knee was taken as specimen.IL-1β,IL-6,and TNF-α in joint fluid were measured by ELISA.The protein expression of Piezo and ERK1/2 in synovial tissue of left knee was measured by Western Blot.The relative mRNA expression of Piezo and ERK1/2 was measured by RT-PCR.Results:The expression levels of Piezo two types and ERK1/2 in the knee synovial tissue of the model group rats were significantly increased compared with the blank group(P<0.05).The content of Piezo two types and ERK1/2 in the synovial tissue of the left knee joint of rats in the model+blocker group was significantly reduced compared with the model group(P<0.05).The expression levels of IL-1β and IL-6 and TNF-α in the joint fluid of the knee joint cavity of the model group rats were significantly increased compared with the blank group(P<0.05).The expression levels of IL-1β and IL-6 and TNF-α in the joint fluid of rats in the knee joint cavity were significantly reduced in both model+TCM washing group and model+TCM washing+blocker group compared with the model group(P<0.05).The expression levels and mRNA levels of Piezo two types and ERK1/2 in the synovial tissue of the left knee joint of the model group rats were sign

关 键 词：机械敏感性离子通道 张氏肌痹洗方 熏洗 机制 

分 类 号：R684.3[医药卫生—骨科学]