WO_(3)纳米片的制备及其在比色测定尿酸中的应用  

Preparation of WO_(3) Nanosheets and Its Application in Colorimetric Detection of Uric Acid

作　　者：曾金敏 吴颜汛 舒馨[1] 廖俊丞 陈万发 郑黄宇 王益林[1] ZENG Jin-min;WU Yan-xun;SHU Xin;LIAO Jun-cheng;CHEN Wan-fa;ZHENG Huang-yu;WANG Yi-in(School of Chemistry and Chemical Engineering of Guangxi University,Guangxi Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《广州化工》2024年第8期38-41,共4页GuangZhou Chemical Industry

基　　金：广西大学大学生创新创业训练计划项目(202110593182);广西高等教育本科教学改革工程项目(2021JGB118)。

摘　　要：在室温条件下制备具有过氧化物酶样活性的WO_(3)纳米片(WO_(3)NSs),基于WO_(3)NSs催化H_(2)O_(2)氧化3,3',5,5'-四甲基联苯胺(TMB)生成蓝色氧化产物(ox-TMB)及在尿酸氧化酶催化下,尿酸被氧化并定量释放H_(2)O_(2)的原理,建立一种以WO_(3)NSs为过氧化物模拟酶的尿酸测定方法。考察了多种因素对吸光度响应的影响。在优化的实验条件下,当尿酸浓度在1.0~100μmol/L范围内时,吸光度与浓度呈良好的线性关系,方法的检出限为0.61μmol/L,应用于血清样品中尿酸的测定,回收率在97.1%~101.4%范围内。WO_(3) nanosheets(WO_(3)NSs)with peroxidase-like activity were prepared at room temperature,which could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine(TMB)by H_(2)O_(2) to produce a blue oxidation product(ox-TMB).Under the catalysis of uric acid oxidase,uric acid is oxidized and H_(2)O_(2) was quantitatively released.Based on of these,a method for the determination of uric acid using WO_(3)NSs as a peroxidase mimic enzyme was established.The effects of various factors on absorbance response were investigated.Under optimized experimental conditions,there was a good linear relationship between absorbance and uric acid concentration in the range of 1.0 to 100μmol/L with a detection limit of 0.61μmol/L.The method exhibits its potential application in the detection of uric acid in serum samples with the recovery of 97.1%to 101.4%.

关 键 词：纳米片 过氧化物模拟酶 比色 尿酸 

分 类 号：O657.3[理学—分析化学]