基于流式细胞术的江西10个油茶农家品种基因组大小测定  

Genome size estimation of 10 farm varieties of Camellia oleifera in Jiangxi Province based on flow cytometry

作　　者：周文才[1] 幸伟年[1] 何小三[1] 温强[1] 左继林[1] Zhou Wencai;Xin Weinian;He Xiaosan;Wen Qiang;Zuo Jilin(Jiangxi Academy of Forestry,Key Laboratory of Cultivation and Utilization of Oil Tea Resources of Jiangxi Province,Nanchang Jiangxi 330013,China)

机构地区：[1]江西省林业科学院油茶资源培育与利用江西省重点实验室,江西南昌330013

出　　处：《南方林业科学》2024年第3期1-4,21,共5页South China Forestry Science

基　　金：江西省重点研发计划项目(项目编号:20224BBF61027);江西省林业局林业专项(项目编号:YCYJZX[2023]111号)。

摘　　要：油茶农家品种具有较高经济价值和地方特色,是油茶育种的重要资源,然而目前有关油茶的遗传基础研究较少。为了探明江西省油茶农家品种的基因组大小,为油茶农家品种的遗传评价与合理利用提供基础,文章采用流式细胞术对江西省10个油茶农家品种的基因组大小进行测定,结果表明,江西油茶农家品种白皮中子、观音桃、茅岗大果、珍珠子、二水桃、霜降红皮、赣萍茶、石市红皮、遂中子、夏讲的基因组大小分别为:5 480.06、8 531.31、8 751.11、8 545.69、8 422.12、8 756.84、8 477.28、8 591.20、8 763.49、8 684.92 Mb,白皮中子的基因组大小接近其他农家品种的2/3,赣石84-8的基因组大小与除白皮中子外的其他油茶农家品种的基因组大小接近。Farm varieties of Camellia oleifera have high economic value and local characteristics,which are important resources for breeding of C.oleifera.At present,there were few researches on the genetic basis of farm varieties of C.oleifera.In order to clarify the genome size of farm varieties of C.oleifera in Jiangxi Province and provide a basis for genetic evaluation and rational utilization of C.oleifera cultivars,the genome size of 10 farm varieties of C.oleifera in Jiangxi Province was determined by flow cytometry.The results showed that the genome sizes of Baipizhongzi,Guanyintao,Maogangdaguo,Zhenzhuzi,Ershuitao,Shuangjianghongpi,Ganpingcha,Shishihongpi,Suizhongzi,and Xiajiang were respectively:5480.06,8531.31,8751.11,8545.69,8422.12,8756.84,8477.28,8591.20,8763.49,8684.92 Mb.The genome size of Baipizhongzi was closed to twothirds of that of other farm varieties,while the genome size of Ganshi 84-8 was similar to that of other farm varieties except for Baipizhongzi.

关 键 词：油茶 农家品种 基因组大小 流式细胞术 

分 类 号：S792.24[农业科学—林木遗传育种]