解冻卵裂期胚胎养成囊胚行植入前遗传学检测二次冷冻复苏移植后临床结局  

Clinical outcomes of frozen-thawed embryo transfer cycles of euploid blastocyst derived from frozen-thawed cleavage embryos

作　　者：李晓芳 师娟子 施文浩 柏海燕 LI Xiao-fang;SHI Juan-zi;SHI Wen-hao;BAI Hai-yan(Assisted Reproduction Center,Northwest Women’s&Children’s Hospital,Xi’an 710003)

机构地区：[1]西北妇女儿童医院生殖中心,西安710003

出　　处：《生殖医学杂志》2024年第7期873-877,共5页Journal of Reproductive Medicine

基　　金：陕西省卫生健康科研创新能力提升计划团队支持项目(2023TD-04);陕西省重点研发计划(2023-ZDLSF-48)。

摘　　要：目的 回顾性评估解冻卵裂期胚胎养成囊胚后进行活检,再次冷冻移植后临床及新生儿结局,以期为临床医生和患者提供参考数据。方法 收集2017年1月至2022年10月于西北妇女儿童医院生殖中心接受解冻卵裂期胚胎养成囊胚后进行活检,再次解冻移植治疗的患者的冻融胚胎移植周期为实验组(n=13),并将女方新鲜周期取卵时年龄、移植囊胚级别、胚胎植入前遗传学诊断的指证以及分类作为匹配因素,按照1∶3配对,选取同周期新鲜囊胚活检、解冻后移植的患者作为对照组(n=39)。比较两组患者的临床基线资料、妊娠结局以及新生儿结局。结果 两组患者临床基本资料、妊娠率以及活产率均无统计学差异(P>0.05),两组产科及新生儿结局亦无显著性差异(P>0.05)。两组患者均未报道重大产科并发症和新生儿畸形。结论 将冻存的卵裂期胚胎解冻后培养至囊胚期进行活检,行胚胎植入前遗传学检测,冷冻后再次复苏行冻融移植周期的妊娠率、活产率及新生儿结局与新鲜囊胚活检后进行冻融移植无明显差异,可以为患者提供更多的选择。Objective:To investigate the clinical and neonatal outcomes of the frozen-thawed embryo transfer cycles of euploid blastocysts derived from frozen-thawed cleavage embryos undergoing blastocyst formation for preimplantation genetic testing(PGT)and re-cryopreservation,in order to provide a basis for clinicians and patients.Methods:Data of patients who underwent blastocyst biopsy and re-thawing transfer after blastocyst formation from thawed cleavage embryos in Reproductive Center of Northwest Women’s&Children’s Hospital from January 2017 to October 2022 were collected as experimental group(n=13).The women’s age at the time of oocyte retrieval,the grade of blastocyst transferred,the indication and category of PGT were taken as matching factors for a 1∶3 pairing.Matched patients with fresh blastocyst biopsy and transfer after thawing in the same period were selected as the control group(n=39).Clinical baseline characteristics,pregnancy and neonatal outcomes were compared between the two groups.Results:Clinical baseline characteristics,clinical pregnancy rate and live birth rate were similar between the two groups(P>0.05).There were no significant differences in obstetric and neonatal outcomes between the two groups(P>0.05).No major obstetrical complications and neonatal malformations were reported in the two groups.Conclusions:There were no significant differences in pregnancy rate,live birth rate and neonatal outcomes between the experimental group and control group,which provided patients with more options.

关 键 词：解冻卵裂期胚胎 胚胎植入前遗传学检测 病例匹配对照研究 活产率 二次冷冻 

分 类 号：R711.6[医药卫生—妇产科学]