基于低共熔策略合成碳纳米聚合物用于多种生物小分子的同时电化学检测  

Synthesis of Carbon Nanopolymers Based on Deep Eutectic Strategy for Simultaneous Electrochemical Detection of a Variety of Biological Small Molecules

作　　者：韦朝鲜 李南盛 庞元昊 张云[1,2] 金文英[2] 袁亚利 WEI Chaoxian;LI Nansheng;PANG Yuanhao;ZHANG Yun;JIN Wenying;YUAN Yali(Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials,Guilin University of Technology,Guilin 541004,China;College of Chemistry and Bioengineering,Guilin University of Technology,Guilin 541004,China)

机构地区：[1]桂林理工大学广西电磁化学功能物质重点实验室,桂林541004 [2]桂林理工大学化学与生物工程学院,桂林541004

出　　处：《高等学校化学学报》2024年第7期37-46,共10页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:22364011)资助.

摘　　要：将基于低共熔策略合成的导电聚合材料聚3,4-乙烯二氧噻吩(PEDOT)与金纳米颗粒(AuNPs)结合,构建了一种新型的电化学传感平台,用于生物小分子抗坏血酸(AA)、多巴胺(DA)和尿酸(UA)的同时测定.对PEDOT和AuNPs复合修饰的玻碳电极(PEDOT-Au/GCE)进行了形貌、性能和有效面积表征.结果表明,该电极可实现对AA,DA和UA高灵敏、高稳定性的同时检测,证明PEDOT-AuNPs具有良好的电催化活性.在最优实验条件下,混合溶液中AA,DA和UA的浓度分别在100~1000,50~500和22.5~225μmol/L范围内与峰电流呈现良好的线性关系,检出限分别为21,0.38和0.016μmol/L.在血清中存在其它活性小分子时,该电化学传感平台对AA,DA和UA具有较高的特异性,且对血清样本中AA,DA和UA的加标回收测定具有较好的回收率.A conductive polymer material poly(3,4-ethylene dioxythiophene)(PEDOT)was synthesized based on a deep eutectic strategy and combined with gold nanoparticles(Au NPs)to construct a novel electrochemical sensing platform to realize the simultaneous determination of a variety of small biological molecules[ascorbic acid(AA),dopamine(DA),uric acid(UA)].The morphology,performance and effective area of glassy carbon electrode modified by PEDOT and Au NPs(PEDOT-Au/GCE)were characterized.The experimental results show that the modified electrode can achieve simultaneous detection of AA,DA and UA with high sensitivity and high stability,proving that the PEDOT-Au NPs complex has good electrochemical catalytic activity.Under optimal experimental conditions,the concentration and peak current value of AA,DA and UA in mixed solution show a good linear relationship in the concentration ranges of 100—1000,50—500 and 22.5—225μmol/L,respectively,with the separate detection limits of 21,0.38 and 0.016μmol/L.In addition,the electrochemical sensing platform has a high specificity for AA,DA,and UA in the presence of other active small molecules in serum.This method is applied for the recovery determination of AA,DA and UA in serum samples.

关 键 词：低共熔策略 聚3 4-乙烯二氧噻吩(PEDOT) 电化学传感器 生物小分子 

分 类 号：O657.1[理学—分析化学]