负载新吲哚菁绿的还原响应性聚合物胶束增强肿瘤光动力治疗  

New indocyanine green-loaded reduction responsive polymeric micelles for enhanced cancer photodynamic therapy

作　　者：胡航 肖文 陶亚宇 杜坤达 袁晓音 徐德锋[1] HU Hang;XIAO Wen;TAO Yayu;DU Kunda;YUAN Xiaoyin;XU Defeng(School of Pharmacy,Changzhou University,Changzhou 213164,China)

机构地区：[1]常州大学药学院,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2024年第4期82-92,共11页Journal of Changzhou University：Natural Science Edition

基　　金：江苏省高等学校自然科学研究面上资助项目(20KJB350005);常州市重点研发计划资助项目(CJ20210030)。

摘　　要：首先合成了二硫键连接的甲氧基聚乙二醇-胆固醇偶联物mPEG5000-SS-CH和mPEG2000-SS-CH,然后通过溶剂扩散法制备了IR820@mPEG5000-SS-CH和IR820@mPEG2000-SS-CH这两种载新吲哚菁绿(New indocyanine green, IR820)胶束,可以显著提高IR820在生理环境下的稳定性。体外药物释放研究显示两种载IR820胶束均具有还原响应性释放特性,细胞摄取研究表明IR820@mPEG2000-SS-CH比游离IR820和IR820@mPEG5000-SS-CH具有更高的细胞摄取量,体外细胞实验显示mPEG5000-SS-CH和mPEG2000-SS-CH具有良好的生物相容性,IR820@mPEG2000-SS-CH比游离IR820和IR820@mPEG5000-SS-CH具有更强的肿瘤细胞光动力杀伤作用。研究制备的IR820@mPEG2000-SS-CH为提高IR820稳定性和肿瘤光动力治疗作用提供了新的选择。In this study,disulfide bonded methoxypolyethylene glycol-cholesterol conjugates mPEG5000-SS-CH and mPEG2000-SS-CH were synthesized.The two types of IR820-loaded micelles,IR820@mPEG5000-SS-CH and IR820@mPEG2000-SS-CH,which might significantly improve the stability of IR820 in physiological environment,were prepared by solvent diffusion method.While vitro studies show that both mPEG5000-SS-CH and mPEG2000-SS-CH have reduction-responsive release characteristics and good biocompatibility,IR820@mPEG2000-SS-CH displays enhanced cellular uptake and photodynamic killing effect on tumor cells as compared to free IR820 and [e-mail] study suggests that IR820@mPEG2000-SS-CH could be a new choice with superior stability and cancer photodynamic therapeutic effect to IR820.

关 键 词：新吲哚菁绿 光动力治疗 聚乙二醇 胆固醇 胶束 还原响应性 

分 类 号：R94[医药卫生—药剂学]