增强UV-B胁迫对小麦幼苗CaM含量的影响  

Impact Calmodulin Content of Enhanced UV-B Radiation Stress on Wheat Seedling

作　　者：张俊红 ZHANG Jun-hong(Linfen Vocational and Technical College,Linfen,Shanxi 041004)

机构地区：[1]临汾职业技术学院,山西临汾041004

出　　处：《园艺与种苗》2024年第5期91-93,共3页Horticulture & Seed

摘　　要：[目的]对增强UV-B胁迫下小麦幼苗中钙调素(CaM)含量进行研究,进而研究增强UV-B胁迫下小麦幼苗体内Ca^(2+)-CaM通路的情况。[方法]采用二次盐析、Sephadex G-50凝胶过滤层析等方法,从小麦幼苗中分离纯化的CaM,并采用PDE法测定CaM含量。[结果]增强UV-B胁迫导致小麦幼苗CaM含量增加了23%,这可能为增强UV-B胁迫导致钙离子增多,进而调控CaM所致。[结论]结果为小麦抗辐射机制的研究奠定了一定的基础。[Objective]To study the Calmodulin content in wheat seedlings by enhanced UV-B radiation stress,and then study the Calcium-Calmodulin signal transduction pathway in wheat seeding after enhanced UV-B Radiation Stress.[Method]The secondary salting method and Sephadex G-50 gel filtration chromatography method were used to separate purify calmodulin from wheat seedling,and the content of calmodulin was determined by PDE method.[Result]The CaM content in wheat seedlings enhanced UV-B radiation stress under normal light levels increased by about 23%。This may be caused by increased calcium ions due to enhanced UV-B stress,which in turn regulates CaM.[Conclusion]The results have laid a certain foundation for the study of the radiation resistance mechanism of wheat.

关 键 词：增强UV-B辐射 CAM 小麦幼苗 PDE法 

分 类 号：S512.1[农业科学—作物学]