红景天提取物对动物皮肤保护作用的研究  

Study on the protective effect of Rhodiola rosea L.extract on animal skin

作　　者：叶佳滨 赵俊钢 张凯妮 付梦丽 王枳懿 韩硕 赵文刚 Ye Jiabin;Zhao Jungang;Zhang Kaini;Fu Mengli;Wang Zhiyi;Han Shuo;Zhao Wen'gang(Wenzhou University,Zhejiang Wenzhou 325035;Children's Hospital of Chongqing Medical University,Chongqing 400014;Medical College of Jinzhou Medical University,Liaoning Jinzhou 121000;Department of Agricultrue and Rural Affairs of LiaoNing Province,Liaoning Shenyang110032)

机构地区：[1]温州大学,浙江温州325035 [2]重庆医科大学附属儿童医院,重庆400014 [3]锦州医科大学医疗学院,辽宁锦州121000 [4]辽宁省农业发展中心,辽宁沈阳110032

出　　处：《现代畜牧兽医》2024年第5期7-12,共6页Modern Journal of Animal Husbandry and Veterinary Medicine

基　　金：辽宁省教育厅基本科研面上项目(JYTMS20230682)。

摘　　要：试验旨在研究红景天提取物(RE)对动物皮肤的保护作用。试验采用紫外线(UVB)辐照诱导角质形成细胞(HaCaT)和小鼠背部皮肤分别建立动物皮肤体外、体内损伤模型。通过检测HaCaT细胞中的DNA损伤、活性氧、炎症因子表达水平和小鼠背部表皮厚度和DNA损伤评估RE对皮肤损伤的保护作用。结果显示,RE可以减少紫外线诱导的HaCaT细胞损伤模型中的DNA损伤,降低细胞中活性氧含量和炎症因子的mRNA表达水平,减少小鼠背部皮肤损伤模型中表皮的厚度和DNA损伤。研究表明,RE对皮肤损伤具有保护作用。The purpose of the experiment was to study the protective effect of RE on animal skin.UVB irradiation was used to induce keratinocytes(HaCaT)and mouse back skin to establish animal skin injury models invitroand invivo.The protective effect of RE on skin injury was evaluated by detecting the levels of DNA damage,reactive oxygen species,inflammatory factors in HaCaT cells,the thickness of mouse dorsal epidermis and DNA damage.The results showed that RE could reduce DNA damage in HaCaT cells induced by ultraviolet rays,reduce the content of reactive oxygen species and mRNA expression of inflammatory factors in cells,and reduce the thickness of epidermis and DNA damage in mouse back skin injury model.Studies have shown that RE has a protective effect on skin injury.

关 键 词：红景天提取物 动物皮肤病 紫外线辐照 皮肤损伤 

分 类 号：S857.5[农业科学—临床兽医学]