分光光度法测定羊毛织物上胶原蛋白的含量  

The spectrophotometric method for quantifying collagen content on wool fabrics

作　　者：赵咪咪 杨瑞婷 王富荣 聂文山 丁慧 徐进 范雪荣 ZHAO Mimi;YANG Ruiting;WANG Furong;NIE Wenshan;DING Hui;XU Jin;FAN Xuerong(School of Textile Science and Engineering,Jiangnan University,Wuxi 214122,China;Baotou Dongbao Biotechnology Co.,Ltd.,Baotou 014030,China;Inner Mongolia Luwang Cashmere Co.,Ltd.,Baotou 014040,China)

机构地区：[1]江南大学纺织科学与工程学院,江苏无锡214122 [2]包头东宝生物技术股份有限公司,内蒙古包头014030 [3]内蒙古鹿王羊绒有限公司,内蒙古包头014040

出　　处：《印染》2024年第7期69-73,共5页China Dyeing and Finishing

基　　金：“科技兴蒙”行动重点专项(XM2023XTGXQ08)。

摘　　要：采用比色法,将胶原蛋白整理的羊毛织物通过酸消解释放L-羟脯氨酸,经氯胺T氧化后,与对二甲氨基苯甲醛反应生成红色化合物,用分光光度计在559 nm处进行检测。试验结果表明:未染色羊毛织物在加热条件下与硫酸反应产生有色物质,对检测结果有一定影响;染色羊毛织物强酸消解时,在强酸和高温环境下织物上分解的染料对检测结果也有一定影响。选取以同质量、同颜色的羊毛织物消解液作为空白样,可基本消除羊毛织物本身及织物上染料对检测结果的影响。L-hydroxyproline is released from the collagen-finished wool fabric through acid digestion,which is oxidized by chloramine T and reacted with p-dimethylaminobenzaldehyde to form red compound.The red com⁃pound is detected by spectrophotometer at 559 nm with colorimetric method.The results show that undyed wool fabrics generate colored substances when subjected to sulfuric acid under heating conditions,which may influence the detection results.Similarly,when dyed wool fabric is digested with strong acid under high tem⁃perature conditions,the decomposition of dyes on the fabric also influences the detection results.Choosing the same quality and color of wool fabric digestion solution as blank sample can basically eliminate the influ⁃ence of wool fabric itself and dye on the detection result.

关 键 词：羊毛织物 强酸消解 胶原蛋白 L-羟脯氨酸 分光光度法 

分 类 号：TS197[轻工技术与工程—纺织化学与染整工程]