IL-1β、IL-6、IFN-γ在甲型H1N1流感病毒性肺炎患儿血清中的表达及其与疾病预后的关系研究  

The expression of IL-1β,IL-6,IFN-γin the serum of children with influenza A(H1N1)virus pneumonia and its correlation with disease prognosis

作　　者：亓伟伟 孙长娜 李燕 QI Wei-wei;SUN Chang-na;LI Yan(Department of Pediatrics,Maternal and Child Health Hospital,Rizhao 276800,China)

机构地区：[1]日照市妇幼保健院儿科,276800

出　　处：《中国实用医药》2024年第12期5-8,共4页China Practical Medicine

摘　　要：目的 研究白细胞介素(IL)-1β、IL-6、γ干扰素(IFN-γ)在甲型H1N1流感病毒性肺炎患儿血清中的表达及其与疾病预后的关系。方法 选取40例甲型H1N1流感病毒性肺炎患儿作为观察组,另选取体检健康儿童40例作为对照组。所有研究对象均行酶联免疫吸附试验(ELISA)法检测IL-1β、IL-6、IFN-γ水平。比较两组IL-1β、IL-6、IFN-γ表达水平;比较IL-1β、IL-6、IFN-γ单一检测与联合检测对甲型H1N1流感病毒性肺炎的诊断效能;比较观察组不同预后(痊愈、有效、无效)患儿IL-1β、IL-6、IFN-γ表达水平。结果 观察组IL-1β、IL-6、IFN-γ表达水平分别为(32.56±3.72)pg/ml、(15.80±3.36)pg/ml、(12.15±3.13)μg/L,均高于对照组的(6.39±1.05)pg/ml、(4.35±0.50)pg/ml、(3.61±0.42)μg/L(P<0.05)。IL-1β对甲型H1N1流感病毒性肺炎的诊断灵敏度为80.00%,特异度为77.50%;IL-6对甲型H1N1流感病毒性肺炎的诊断灵敏度为75.00%,特异度为77.50%;IFN-γ对甲型H1N1流感病毒性肺炎的诊断灵敏度为77.50%,特异度为80.00%;联合检测对甲型H1N1流感病毒性肺炎的诊断灵敏度为95.00%,特异度为55.00%;联合检测对甲型H1N1流感病毒性肺炎的诊断灵敏度均高于IL-1β、IL-6、IFN-γ单一检测,特异度低于IL-1β、IL-6、IFN-γ单一检测(P<0.05)。观察组患儿治疗后痊愈18例,有效17例,无效5例。其中痊愈患儿IL-1β、IL-6、IFN-γ表达水平均低于有效及无效患儿,有效患儿IL-1β、IL-6、IFN-γ表达水平均低于无效患儿(P<0.05)。结论 IL-1β、IL-6、IFN-γ与甲型H1N1流感病毒性肺炎患儿病情有关,上述指标对判断患儿预后有着重要价值。Objective To study the expression of interleukin-1β(IL-1β),interleukin-6(IL-6),gamma interferon(IFN-γ)in the serum of children with influenza A(H1N1)virus pneumonia and its correlation with disease prognosis.Methods 40 children with influenza A(H1N1)virus pneumonia were selected as the observation group,and another 40 healthy children who underwent physical examinations were selected as the control group.All research subjects were tested for IL-1β,IL-6 and IFN-γusing enzyme linked immunosorbent assay(ELISA)method.Comparison was made on expressions of IL-1β,IL-6 and IFN-γbetween the two groups,diagnostic efficacy of single versus combined testing of IL-1β,IL-6 and IFN-γfor influenza A(H1N1)virus pneumonia,as well as the expression levels of IL-1β,IL-6,and IFN-γin children with different prognoses(cured,effective,and ineffective)in the observation group.Results The expression levels of IL-1β,IL-6 and IFN-γin the observation group were(32.56±3.72)pg/ml,(15.80±3.36)pg/ml and(12.15±3.13)μg/L,which were higher than(6.39±1.05)pg/ml,(4.35±0.50)pg/ml and(3.61±0.42)μg/L in the control group(P<0.05).IL-1βhad a diagnostic sensitivity of 80.00%and a specificity of 77.50%for influenza A(H1N1)virus pneumonia;IL-6 had a diagnostic sensitivity of 75.00%and a specificity of 77.50%for influenza A(H1N1)virus pneumonia;IFN-γhad a diagnostic sensitivity of 77.50%and a specificity of 80.00%for influenza A(H1N1)virus pneumonia;combined testing has a diagnostic sensitivity of 95.00%,and a specificity of 55.00%.The diagnostic sensitivity of the combined testing for influenza A(H1N1)virus pneumonia was higher than that of the single testing of IL-1β,IL-6 and IFN-γ,and the specificity was lower than that of the single testing of IL-1β,IL-6 and IFN-γ(P<0.05).In the observation group,18 children were cured after treatment,17 were effective and 5 were ineffective.The expression levels of IL-1β,IL-6 and IFN-γin the cured children were lower than those in the effective and ineffective children,and the expression leve

关 键 词：白细胞介素-1Β 白细胞介素-6 Γ干扰素 甲型H1N1流感病毒性肺炎 疾病预后 

分 类 号：R725.1[医药卫生—儿科]