眼络通方对大鼠视网膜静脉阻塞模型ET-1/ETAR信号通路的影响  

The effect of Yanluotong on the ET-1/ETAR signaling pathway in a rat retinal vein occlusion model

作　　者：朱永唯[1] 朱懿行 翁文庆[1] 熊烈 吴轶波 和艳艳 蒋丽君[1] 冯燕兵 Zhu Yongwei

机构地区：[1]浙江省嘉兴市中医医院,314000

出　　处：《浙江临床医学》2024年第6期810-813,共4页Zhejiang Clinical Medical Journal

基　　金：浙江省中医药科技计划项目(2021ZB284,2023ZR053);嘉兴市科技计划项目(2021AY30007)。

摘　　要：目的探讨眼络通对大鼠视网膜静脉阻塞(RVO)模型ET-1/ETAR信号通路的影响。方法将48只健康雄性SD大鼠随机分为空白对照组(Control),模型对照组(Model)、波生坦组(Bosentan)、眼络通组(Yanluotong),以光化学法建立RVO模型SD大鼠各12只,连续给药3周。在第3周时处死SD大鼠,取血清用于Elisa检测,取视网膜组织用于荧光定量PCR与免疫荧光检测,测定其中ET-1、ETAR、VEGF、TNF-α、IL-6蛋白与mRNA表达水平。结果Elisa检测发现模型对照组血清IL-6、TNF-α、VEGF较空白对照组升高(P<0.01),波生坦和眼络通方干预3周后比模型对照组均下降(P<0.01);免疫荧光显示相对于空白对照组,RVO模型对照组ET-1、IL-6、TNF-α、VEGF表达量均增多,波生坦和眼络通方治疗后表达量均呈下降趋势。对于ET-1、ETAR、VEGF mRNA表达量,模型对照组较空白对照组上调(P<0.01),波生坦和眼络通方均能下调上述基因mRNA的表达(P<0.01)。结论ET-1/ETAR信号通路在RVO发病过程中被异常激活,眼络通方可通过ET-1/ETAR信号通路发挥抑制视网膜血管收缩、减轻炎症反应、抑制VEGF作用,干预RVO病情进展。Objective To explore the effect of Yanluotong on the ET-1/ETAR signaling pathway in a rat retinal vein occlusion(RVO)model.Methods 48 healthy male SD rats were randomly divided into a blank control group(Control),a model control group(Model),a Bosentan group,and a Yanluotong group.Twelve RVO model SD rats were established using photochemical methods and administered continuously for three weeks.At the third week,SD rats were euthanized and serum was collected for Elisa detection.Retinal tissue was taken for fluorescence quantitative PCR and immunofluorescence detection,and the protein and mRNA expression levels of ET-1,ETAR,VEGF,and TNF-α,IL-6 were measured.Results The level of serum IL-6,TNF-αand VEGF in the model group were significantly increased compared to the blank control group(P<0.01),while after intervention with Bosentan and Yanluotong Formula for 3 weeks,VEGF levels were significantly decreased compared to the model group(P<0.01).Immunofluorescence showed that the expression level of ET-1,IL-6,TNF-αand VEGF increased in the RVO model group compared to the normal blank group,but after treatment with Bosentan and Yanluotong Formula,the expression level showed a downward trend.For the mRNA expression levels of ET-1,ETAR,and VEGF in the model control group was significantly upregulated compared to the blank control group(P<0.01),and the use of bosentan and yanluotong formula could significantly downregulate the mRNA expression of these genes(P<0.01).Conclusion The ET-1/ETAR signaling pathway is abnormally activated during the pathogenesis of RVO.Yanluotong can exert inhibitory effects on retinal vasoconstriction,alleviate inflammatory response,inhibit VEGF,and intervene in the progression of RVO through the ET-1/ETAR signaling pathway.

关 键 词：眼络通 视网膜静脉阻塞 内皮素-1 内皮素受体A 炎性因子 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]