孕期病原菌感染和叶酸代谢相关基因多态性与胚胎停止发育的关联  

Association of gestational pathogens infections,polymorphisms of folic acid metabolism-related genes with embryonic arrest

作　　者：姜素娥 谢艳[1] 朱晓琴[1] 韩雪芹[1] JIANG Su-e;XIE Yan;ZHU Xiao-qin;HAN Xue-qin(Huai'an Maternal and Child Health Hospital,Huai'an,Jiangsu 223002,China)

机构地区：[1]淮安市妇幼保健院孕期保健科,江苏淮安223002

出　　处：《中华医院感染学杂志》2024年第11期1740-1744,共5页Chinese Journal of Nosocomiology

基　　金：江苏省卫生健康委科研项目(ZD2021044)。

摘　　要：目的探究孕期病原菌感染和叶酸代谢相关基因多态性与胚胎停育的关系.方法选取2021年10月-2022年12月淮安市妇幼保健院孕期保健科收治的102例胚胎停育孕妇(记为胚胎停育组)和同期于医院接受常规、产检的孕妇45例(无不良妊娠结局,记为对照组)为研究对象,检测两组孕妇病原学、叶酸代谢相关基因多态性,分析其与胚胎停育的关联性.结果胚胎停育组风疹病毒、巨细胞病毒免疫球蛋白M(IgM)阳性率较对照组高(P<0.05);胚胎停育组叶酸代谢相关基因MTH-FR C677T中TT基因型、T等位基因和MTH-FR A1298C中CC基因型、C等位基因及MTRR A66G中G等位基因分布频率高于对照组明(P<0.05);MTH-FR C677T中TT基因型、T等位基因和MTH-FR A1298C中CC基因型、C等位基因及MTRR A66G中G等位基因与胚胎停育具有相关性(P<0.05);胚胎停育孕妇MTH-FR C677T显性模型和MTRR A66G显性模型与胚胎停育相关(P<0.05);病原菌感染(风疹病毒、巨细胞病毒IgM阳性)和叶酸代谢位点多态性(MTH-FR C677T和MTRR A66G位点)是胚胎停育的危险因素(P<0.05).结论病原学、叶酸代谢MTH-FR C677T和MTRR A66G基因多态性与胚胎停育有关,可通过孕前检测叶酸代谢相关基因多态性和病原菌感染情况评估孕妇胚胎停育发生风险.OBJECTIVE To investigate the association of gestational pathogens infections,polymorphisms of folic acid metabolism-related genes with embryonic arrest.METHODS A total of 102 pregnant women with embryonic arrest(recorded as the embryonic arrest group)who were admitted to the Antenatal Care Department of Huai'an Maternal and Child Health Hospital from October 2021 to December 2022,and 45 pregnant womenwho received routine pregnancy and antenatal care in the same hospital during the same period(with no adverse pregnancy outcome,recorded as the control group)were selected as the research subjects.Pathogens and polymorphisms of folic acid metabolism-related genes in the two groups were detected,and their association with embryonic arrest was analyzed.RESULTS The immunoglobulin M(IgM)positive rates of rubella virus and cytomegalovirus in the embryonic arrest group were higher than those in the control group(P<o.05).The frequencies of TT genotype and T allele of folic acid metabolism-related gene MTH-FR C677T,CC genotype and C allele of MTH-FR A1298C,and G allele of MTRR A66G in the embryonic arrest group were higher than those in the control group(P<0.05).TT genotype and T allele of MTH-FR C677T,CC genotype and C allele of MTH-FR A1298C,and G allele of MTRR A66G were related to the occurrence of embryonic arrest(P<0.05).The MTH-FR C677T dominant model and the MTRR A66G dominant model were related to embryonic arrest(P<0.05).Pathogenic bacterial infection(IgM positive rates of rubella virus and cytomegalovirus)and polymorphisms of folic acid metabolism-related loci(MTH-FR C677T and MTRR A66G)were risk factors for embryonic arrest(P<0.05).CONCLUSION Etiology and polymorphisms of folic acid metabolism-related genes(MTH-FR C677T and MTRR A66G)are related to embryonic arrest.The risk of developing embryonic arrest can be assessed by pre-pregnancy testing for polymorphisms of folic acid metabolism-related genes and pathogenic bacterial infection.

关 键 词：胚胎停育 病原菌感染 叶酸代谢 基因多态性 关联性 

分 类 号：R714.431[医药卫生—妇产科学]