四君子汤调控AMPK-SIRT1蛋白改善运动性疲劳小鼠学习记忆的机制研究  

Study on Mechanism of Sijunzi Decoction in Regulating AMPK-SIRT1 Protein for Improving Learning and Memory of Exercise-Induced Fatigue Mice

作　　者：庞贤妹[1] 耿雪 陈楚杰[1] 陈道睿 刘刚[1] PANG Xianmei;GENG Xue;CHEN Chujie;CHEN Daorui;LIU Gang(School of Physical Education&Health,Guangzhou University of Chinese Medicine,Guangzhou Guangdong 510006,China;Guangzhou City Construction College,Guangzhou Guangdong 510925,China)

机构地区：[1]广州中医药大学体育健康学院,广东广州510006 [2]广州城建职业学院,广东广州510925

出　　处：《新中医》2024年第11期23-28,共6页New Chinese Medicine

基　　金：广东省中医药局项目(20211113)。

摘　　要：目的:观察四君子汤对运动性疲劳(EF)小鼠AMPK/SIRT1信号通路的影响,并探讨其改善EF认知损伤和学习记忆能力的机制。方法:将60只C57BL/6小鼠随机分为空白组、模型组、四君子汤高剂量组、四君子汤中剂量组、四君子汤低剂量组。除空白组外,其他各组小鼠构建脾气虚证EF小鼠模型。四君子汤高、中、低剂量组小鼠分别给予不同浓度的四君子汤灌胃,空白组和模型组以等体积蒸馏水灌胃,各组连续灌胃4周。采用Morris水迷宫实验评估小鼠的认知功能,采用ELISA法检测小鼠血清中乳酸、血糖、肝糖原、肌糖原的水平,Western Blot法检测小鼠脑组织中AMPK和SIRT1的蛋白表达量。结果:实验后,模型组小鼠体质量、摄食量、血糖、肝糖原含量、肌糖原含量、目标象限游泳时间、穿越平台次数、AMPK蛋白表达量、SIRT1蛋白表达量低于空白组,血清乳酸水平高于空白组(P<0.05)。给药干预后,四君子汤高、中、低剂量组小鼠体质量、摄食量、力竭游泳时间、肝糖原含量、肌糖原含量、目标象限游泳时间、穿越平台次数高于模型组,血清乳酸水平低于模型组(P<0.05)。四君子汤高剂量小鼠游泳力竭时间高于低剂量组,乳酸水平低于低剂量组(P<0.05)。四君子汤高、中剂量组肝糖原、肌糖原含量高于低剂量组(P<0.05)。与模型组比较,四君子汤高、中剂量组AMPK及SIRT1蛋白表达量增多(P<0.05),四君子汤低剂量组SIRT1蛋白表达量增多(P<0.05)。与四君子汤低剂量组比较,四君子汤高、中剂量组AMPK及SIRT1蛋白表达量增多(P<0.05)。结论:四君子汤可在一定程度调控AMPK/SIRT1的活性,进而改善EF小鼠脾虚症状。Objective:To observe the effect of Sijunzi Decoction on the AMPK-SIRT1 signal pathway of exercise-induced fatigue(EF)mice,and discuss its mechanism in improving EF cognitive injury and learning and memory function.Methods:A total of 60 C57BL/6 mice were randomly divided into the blank group,the model group,the high-dose Sijunzi Decoction group,the medium-dose Sijunzi Decoction group,and the low-dose Sijunzi Decoction group.Except for the blank group,the other groups were used to establish the EF mice model with syndrome of spleen-qi deficiency.The high-dose Sijunzi Decoction group,the medium-dose Sijunzi Decoction group,and the low-dose Sijunzi Decoction group were given Decoction of different concentrations by gavage,and the blank group and the model group were given equal volume of distilled water by gavage.Each group was given gavage for four weeks.Morris water maze test was used to evaluate the cognitive function of mice,ELISA method was used to detect the levels of serum lactic acid,blood glucose,liver glycogen and muscle glycogen and Western Blot method was used to detect the protein expressions of AMPK and SIRT1 in brain tissue.Results:After experiment,the body mass,food intake,blood glucose,liver glycogen content,muscle glycogen content,target quadrant swimming time,number of crossing platforms,and protein expressions of AMPK and SIRT1 in the model group were lower than those in the blank group,and the level of serum lactic acid was higher than that in the blank group(P<0.05).After intervention with drugs,the body mass,food intake,exhaustive swimming time,liver glycogen content,muscle glycogen content,target quadrant swimming time and number of crossing platform in the high-dose Sijunzi Decoction group,the medium-dose Sijunzi Decoction group,and the low-dose Sijunzi Decoction group were higher than those in the model group,and the levels of serum lactic acid were lower than those in the model group(P<0.05).The exhaustive swimming time in the high-dose Sijunzi Decoction group was higher than that in the lo

关 键 词：运动性疲劳 脾气虚证 四君子汤 AMPK/SIRT1蛋白 乳酸 糖原 

分 类 号：R285.5[医药卫生—中药学]