LncRNA TPTEP1/miR-137/KLF15轴在肥胖相关性肾病中的作用机制研究  

The Mechanism of Action of LncRNA TPTEP1/miR-137/KLF15 Axis in Obesity Associated Nephropathy

作　　者：薛正彪 钟恒泉 吴超瑜 郭宗文 谢江波 XUE Zhengbiao;ZHONG Hengquan;WU Chaoyu;GUO Zongwen;XIE Jiangbo(First Affiliated Hospital of Gannan Medical University,Jiangxi Ganzhou 341000)

机构地区：[1]赣南医科大学第一附属医院,江西赣州341000

出　　处：《深圳中西医结合杂志》2024年第10期7-10,I0003,I0004,共6页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：赣州市科技计划项目(GZ2023ZSF100)。

摘　　要：目的:探讨长链非编码核糖核酸(LncRNA)人肌力蛋白磷酸酶同源假基因1(TPTEP1)/微小核糖核酸-137(miR-137)/Kruppel样因子15(KLF15)轴在肥胖相关性肾病(ORKD)中的作用机制。方法:选择赣南医科大学第一附属医院2022年2月至2024年1月20例ORKD住院者为ORKD组,同期住院的非ORKD患者20例为对照组,采集血液进行生化检验,通过免疫组化及电镜检查肾活检标本病理变化,利用生物信息学工具对比分析ORKD组织与正常肾组织中KLF15信使核糖核酸(mRNA)的表达水平,酶联免疫吸附试验分析血清脂联素、瘦素水平。结果:ORKD组患者的血肌酐(Scr)、尿酸(UA)、24 h尿蛋白定量(24h-UTP)水平高于对照组,差异均具有统计学意义(P<0.05);ORKD组肾组织KLF15表达水平、血清脂联素水平相比于对照组明显降低,瘦素水平明显增加,差异均具有统计学意义(P<0.05);高通量测序筛查LncRNA发现,ORKD组ENSG00000100181.15表达差异大,miR-137可能是LncRNA TPTEP1的靶基因,LncRNA TPTEP1在机体多种组织中均有表达,其中包括肾脏及脂肪组织。结论:ORKD的肾组织中KLF15和脂联素的异常可能与疾病的发展有关,LncRNA TPTEP1/miR-137/KLF15轴在ORKD发展过程中发挥着重要的作用。Objective To investigate the mechanism of long non-coding ribonucleic acid(LncRNA)human myodynamin phosphatase homologous pseudogene 1(TPTEP1)/microrna-137(miR-137)/Kruppel-like factor 15(KLF15)axis in obesity-related nephropathy(ORKD).Methods A total of 20 patients with ORKD hospitalized from the First Affiliated Hospital of Gannan Medical University from February 2022 to January 2024 were selected as the ORKD group,and 20 patients with non-ORKD hospitalized during the same period were selected as the control group.Blood samples were collected for biochemical examination,and pathological changes of renal biopsy specimens were examined by immunohistochemistry and electron microscopy.The bioinformatics tools were used to compare and analyze the expression levels of KLF15 messenger ribonucleic acid(mRNA)in ORKD tissues and normal renal tissues,and enzyme-linked immunosorbent assay was used to analyze serum adiponectin and leptin levels.Results The levels of serum creatinine(Scr),uric acid(UA)and 24h urinary protein(24H-UTP)in ORKD group were higher than those in the control group,with statistical significances(P<0.05).Compared with the control group,the expressions of KLF15 in renal tissue and serum adiponectin level in the ORKD group were significantly reduced compared to the control group,while the level of leptin was significantly increased,and the differences were statistically significant(P<0.05).After LncRNA screening by high-throughput sequencing,it was found that the expression of ENSG00000100181.15 in ORKD group was significantly different,and miR-137 may be the target gene of LncRNA TPTEP1,which was expressed in various tissues of the body,including kidney and adipose tissue.Conclusion The abnormal function of KLF15 in ORKD renal tissue and adiponectin may be related to the development of the disease,and the LncRNA TPTEP1/miR-137/KLF15 axis plays an important role in the development of ORKD.

关 键 词：肥胖相关性肾病 人肌力蛋白磷酸酶同源假基因1 微小核糖核酸-137 Kruppel样因子15 长链非编码核糖核酸 

分 类 号：R692.9[医药卫生—泌尿科学]