‘午夜’紫薇离体茎段快繁体系的建立  

Establishment of a Rapid Propagation System for in vitro Stem Segments of Lagerstroemia indica‘Wuye’

作　　者：马清清 焦紫薇 沈鸿明 王国联 赵雨 顾翠花[1,2,3] MA Qingqing;JIAO Ziwei;SHEN Hongming;WANG Guolian;ZHAO Yu;GU Cuihua(College of Landscape and Architecture,Zhejiang A&F University,Hangzhou,Zhejiang 311300;Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants,Zhejiang A&F University,Hangzhou,Zhejiang 311300;Key Laboratory of National Forestry and Grassland Administration on Germplasm Innovation and Utilization for Southern Garden Plants,Zhejiang A&F University,Hangzhou,Zhejiang 311300;Zhejiang Sencheng Seed Industry Limited Company,Haining,Zhejiang 314406)

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江杭州311300 [2]浙江农林大学浙江省园林植物种质创新与利用重点实验室,浙江杭州311300 [3]浙江农林大学南方园林植物种质创新与利用国家林业和草原局重点实验室,浙江杭州311300 [4]浙江森城种业有限公司,浙江海宁314406

出　　处：《北方园艺》2024年第12期52-58,共7页Northern Horticulture

基　　金：浙江省教育厅科研资助项目(Y202250160);浙江省自然科学基金资助项目(LY21C160001);浙江省农业新品种重大专项花卉育种专项资助项目(2021C02071-4)。

摘　　要：以紫薇新品种‘午夜’带腋芽的幼嫩茎段为外植体,采用正交实验法,研究了NaClO与C_(2)H_(5)OH不同组合的消毒时间对外植体污染及萌发的影响,探讨WPM、1/2MS及MS 3种基本培养基和6-BA、NAA不同浓度组合对‘午夜’紫薇嫩茎腋芽诱导和增殖的影响以及在3种基本培养基上和不同IBA浓度组合对‘午夜’紫薇生根的影响,以期为‘午夜’紫薇大量繁殖提供参考依据。结果表明:75%C_(2)H_(5)OH消毒时间为30 s,5%NaClO消毒时间为6 min时,污染率较低,为18.6%;WPM培养基适合‘午夜’紫薇的茎段腋芽诱导与增殖,当6-BA、NAA浓度分别为1.0、0.5 mg·L^(-1)时诱导率最高,达93.28%;当6-BA浓度为1.0 mg·L^(-1),NAA浓度为0.1 mg·L^(-1)时的增殖系数最高,为5.83;IBA浓度变化对‘午夜’紫薇生根影响显著,在1/2MS培养基中添加0.5 mg·L^(-1)IBA时生根率最高,为92.99%,且无菌苗生长情况较好;炼苗后将组培苗移栽到泥炭土∶珍珠岩∶蛭石为3∶1∶2的基质上,成活率高达91%。Taking the young stem segments with axillary buds of a new variety of Lagerstroemia indica‘Wuye’as explants,the orthogonal experimental method was used to study the effects of disinfection time of different combinations of NaClO and C2H5OH on the contamination and germination of the explants.The effects of three basic media,WPM,1/2MS,and MS,as well as different concentration combinations of 6-BA and NAA,on the induction and proliferation of axillary buds from tender stems of L.indica‘Wuye’were studied,as well as the effects of three basic media and different concentration combinations of IBA on the rooting of L.indica‘Wuye’were studied,in order to provide reference for mass propagation of Lagerstroemia indica‘Wuye’.The results showed that,when the disinfection time of 75%C2H5OH was 30 seconds and the disinfection time of 5%NaClO was 6 minutes,the pollution rate was lower,at 18.6%.The WPM medium was suitable for the induction and proliferation of axillary buds in stem segments of L.indica‘Wuye’.The highest induction rate was achieved when the concentrations of 6-BA and NAA were 1.0 mg·L^(-1) and 0.5 mg·L^(-1),respectively,reaching 93.28%.When the concentration of 6-BA was 1.0 mg·L^(-1) and the concentration of NAA was 0.1 mg·L^(-1),the highest proliferation coefficient was 5.83.The concentration change of IBA had a significant impact on the rooting of L.indica‘Wuye’.Adding 0.5 mg·L^(-1) IBA to the 1/2MS had the highest rooting rate of 92.99%,and the growth of sterile seedlings was good.After refining the seedlings,transplanted the tissue culture seedlings onto a substrate of peat soil∶perlite∶vermiculite in a ratio of 3∶1∶2,with a survival rate of up to 91%.

关 键 词：‘午夜’紫薇 茎段 腋芽诱导 增殖 生根 

分 类 号：S723.1[农业科学—林木遗传育种]