继代次数对黑木耳菌种活力的影响  

Effects of Subculture Times on the Viability of Auricularia heimuer Strains

作　　者：和耀威 黄静 李鹏[1] 刘忠玄 向准[1] 谭静 HE Yaowei;HUANG Jing;LI Peng;LIU Zhongxuan;XIANG Zhun;TAN Jing(Guizhou Institute of Biology,Guizhou Academy of Sciences,Guiyang,Guizhou 550009;Qujing Academy of Agricultural Sciences,Qujing,Yunnan 655000)

机构地区：[1]贵州科学院贵州省生物研究所,贵州贵阳550009 [2]曲靖市农业科学院,云南曲靖655000

出　　处：《北方园艺》2024年第12期115-121,共7页Northern Horticulture

基　　金：贵州省科技计划资助项目(黔科合重大专项字[2019]3007-5号,黔科合成果[2020]1Z003号,黔科合支撑[2021]一般195);贵州省特色林业产业科研资助项目(贵[2022]TSLY06)。

摘　　要：以黑木耳“吉蕈-A8”为试材,对其进行继代培养,研究继代次数对菌丝生长特性、显微特征、胞外酶活性、遗传变异及农艺性状等菌种活力指标的影响,以期为菌种退化与继代次数相关性研究提供参考依据。结果表明:菌种在继代15次内能保持遗传稳定性;但继代培养会使菌丝变得更加细长,分支减少;F_(1)~F_(7)区间整体活力高,在F7代左右生长速率更快,漆酶、淀粉酶和蛋白酶活性能维持较高水平,菌丝尖端粗壮,分支多且密,与最终农艺性状呈正相关。在生产中要确保黑木耳菌种高活力,继代次数应控制在3~6次。Taking Auricularia heimuer‘Jixun-A8’as the test material,it was subcultured to study the effect of subculture times on the mycelial growth characteristics,microscopic characteristics,extracellular enzyme activity,genetic variation and agronomic traits of the strain,in order to provide reference for the study of the correlation between strain degradation and subculture times.The results showed that the strain could maintain genetic stability within 15 generations;however,subculture would make the mycelia more slender and the branches fewer.The overall vitality was high in the F_(1)-F_(7)interval,with a faster growth rate around the F_(7)generation,higher laccase,amylase and protease activities,thicker and denser hyphal tips,and more branches,which were positively correlated with the final agronomic traits.In production,to ensure the high vitality of Auricularia heimuer strains,the number of subcultures should be controlled within 3-6 times.

关 键 词：黑木耳 继代次数 菌种活力 酶活性 农艺性状 

分 类 号：S646[农业科学—蔬菜学]