湖南产山橿乙酸乙酯部位化学成分及抗肿瘤活性研究  

Study on the chemical constituents and antitumor activity of ethyl acetate extract of Lindera reflexa from Hunan province

作　　者：张珊珊[1,2] 韩月 侯亚迪 魏雨婕 孙孝亚 陈随清[1,2,3] ZHANG Shan-shan;HAN Yue;HOU Ya-di;WEI Yu-jie;SUN Xiao-ya;CHEN Sui-qing(Henan University of Chinese Medicine,Zhengzhou 450046,China;Henan Key Laboratory of Chinese Medicine Resources and Chinese Medicine Chemistry,Zhengzhou 450046,China;Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province&Education Ministry of P.R.China,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中药资源与中药化学重点实验室,河南郑州450046 [3]呼吸疾病中医药防治省部共建协同创新中心,河南郑州450046

出　　处：《药学学报》2024年第6期1741-1750,共10页Acta Pharmaceutica Sinica

基　　金：河南省科技攻关项目(212102310353);国家自然科学基金资助项目(81773859).

摘　　要：采用硅胶、MCI、Sephadex LH-20及半制备高效液相等色谱技术进行分离纯化,并根据NMR、MS等波谱数据确定化合物结构,从湖南产山橿乙酸乙酯部位分离得到20个单体化合物,分别鉴定为:(1″S,3″S,6″R)-2′,4′,6′-三羟基-3′-[1″-(3″-羟基)-对薄荷烷基]-查尔酮(1)、sumadain D(2)、槲皮素(3)、儿茶素(4)、表儿茶素(5)、N-顺式阿魏酰酪胺(6)、N-反式阿魏酰酪胺(7)、N-反式-阿魏酰-3-甲氧基酪胺(8)、flavifloramides B(9)、northalifoline(10)、异落叶松脂素(11)、丁香树脂酚(12)、松脂素(13)、皮树脂醇(14)、二氢去氢二愈创木基醇(15)、4-methoxyl-denudaquinol(16)、miliusanal(17)、丁香酸(18)、脱落酸(19)、桂酸桂酯(20)。化合物1为新化合物,化合物2~20首次从山橿中分离得到。抗肿瘤活性筛选结果表明,化合物1、2、3、16可以显著降低胃癌细胞MGC-803的存活能力,其IC50值分别为5.58、23.41、25.72、20.96μmol·L^(-1)。化合物5、20可以降低胃癌细胞MGC-803的存活能力,IC50值分别为98.83、89.26μmol·L^(-1)。The compounds were isolated and purified by silica gel,MCI,Sephadex LH-20 and semipreparative high performance liquid chromatography.The structures of the compounds were determined by NMR and MS spectroscopic data.Twenty monomer compounds were isolated from the ethyl acetate extract of Lindera reflexa root from Hunan province,and identified as:(1″S,3″S,6″R)-2′,4′,6′-trihydroxy-3′-[1″-(3″-hydroxy)-pmenthanyl]-chalcone(1),sumadain D(2),quercetin(3),catechin(4),epicatechin(5),N-cis-feruloyltyramine(6),Ntrans-feruloyltyramine(7),N-trans-feruloyl-3-methoxytyramine(8),flavifloramides B(9),northalifoline(10),isolariciresinol(11),syringaresinol(12),pinoresinol(13),medioresinol(14),dehydroconiferyl alcohol(15),4-methoxyl-denudaquinol(16),miliusanal(17),syringic acid(18),abscisic acid(19),(E)-cinnamyl-(E)-cinnamate(20).Compound 1 is a new compound,and compounds 2‒20 have been isolated from Lindera reflexa for the first time.The results showed that compounds 1,2,3 and 16 could significantly reduce the survival ability of MGC-803 cells with IC50 values of 5.58,23.41,25.72 and 20.96μmol·L^(-1),respectively.Compounds 5 and 20 can reduce the survival ability of MGC-803 cells with IC50 values of 98.83 and 89.26μmol·L^(-1),respectively.

关 键 词：山橿 抗肿瘤活性 黄酮 酰胺 木脂素 

分 类 号：R284[医药卫生—中药学]