滇黄精全长转录组测序及生物信息学分析  

Full-length transcriptome sequencing and bioinformatics analysis of Polygonatum kingianum

作　　者：米琪 赵艳丽 徐萍 于梦雯 张萱 涂振华 李春华 郑国伟 陈佳 MI Qi;ZHAO Yan-li;XU Ping;YU Meng-wen;ZHANG Xuan;TU Zhen-hua;LI Chun-hua;ZHENG Guo-wei;CHEN Jia(College of Chinese Material Medica,Yunnan University of Chinese Medicine,Kunming 650500,China;College of Ethnic Medicines,Yunnan University of Chinese Medicine,Kunming 650500,China;Gongshan County Agriculture Village Ju Agriculture Comprehensive Development Service Center,Nujiang of the Lisu Autonomous Prefecture,Nujiang 673500,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南中医药大学民族医药学院,云南昆明650500 [3]贡山县农业农村局农业综合发展服务中心,云南怒江傈僳族自治州673500

出　　处：《药学学报》2024年第6期1864-1872,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82360751);云南中医药大学高层次人才计划(2019YZG07);云南青年精英人才项目(YNWR-QNBJ-2020-289).

摘　　要：本文通过PacBio Sequel平台对滇黄精根茎进行全长转录组测序,并对测序结果进行生物信息学分析,旨在为滇黄精基因功能的研究提供参考。最终测得polymerase reads为1120485个,总数据共77.73 GB。在NR数据库中,有41864个同源序列比对到5个物种;有40506个在KOG数据库得到注释,根据其功能分为26类;有69060个得到GO注释,分为细胞组分、分子功能及生物学过程等3大类的32个功能组;有45779个注释到KEGG数据库的145条代谢途经中,其中鉴定与多糖合成有关的转录本在糖酵解/糖异生代谢通路中有127条,淀粉和蔗糖代谢通路有144条,氨基酸糖和核苷酸糖代谢通路有85条,果糖和甘露糖代谢通路有69条;此外还检测到1781个转录因子,分布在37个转录因子家族中;检测到了180293个SSR位点,其中以单碱基重复占比最多,占所有碱基重复类型的30.48%,最少的是五碱基重复,仅为单碱基重复的0.14%。本研究结果为丰富滇黄精基因组信息,探索其有效成生物合成途径提供重要的数据支持。The purpose of this study was to enrich the genomic information and provide a basis for further development and utilization of Polygonatum kingianum.The fresh rhizome of P.kingianum was used as the experimental material,and the full-length transcriptome was sequenced by PacBio Sequel platform.The final measured polymerase reads were 1120485,with a total of 77.73 GB of data.In NR database,41864 homologous sequence alignments were aligned to 5 species;40506 were annotated in the KOG database and classified into 26 categories based on their functionality;69060 GO annotated 32 functional groups divided into 3 categories:cellular components,molecular functions,and biological processes;45779 annotations were added to 145 metabolic pathways in the KEGG database.Among them,there are 127 identified transcripts related to polysaccharide synthesis in the glycolysis/gluconeogenesis metabolic pathway,144 in the starch and sucrose metabolic pathway,85 in the amino acid sugar and nucleotide sugar metabolic pathway,and 69 in the fructose and mannose metabolic pathway.In addition,1781 transcription factors were detected,distributed in 37 transcription factor families;180293 SSR loci were detected,among which single base repeats accounted for the most,accounting for 30.48%of all base repeats,and at least five base repeats,accounting for only 0.14%of single base repeats.The results of this study provide important data supporting for enriching the genomic information of P.kingianum and exploring its effective biosynthetic pathway.

关 键 词：滇黄精 全长转录组 种质资源 多糖合成 

分 类 号：R931[医药卫生—生药学]