不同生境黄柳叶片渗透调节物质含量及抗氧化酶活性研究  

Study on the Osmoregulatory Substances Content and Antioxidiant Enzymes activities of Salix gordejevii in Different Habitats

作　　者：刘冠志 李梓豪 张艳成 张世琦 马少薇 兰庆 刘果厚 LIU Guanzhi;LI Zihao;ZHANG Yancheng;ZHANG Shiqi;MA Shaowei;LAN Qing;LIU Guohou(College of Grassland,Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010019,China;National Demonstration Center for Experimental Botany Education,Inner Mongolia,Hohhot 010019,China;Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resource,Hohhot 010018,China;Key Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region,Hohhot 010011,China;Inner Mongolia Academy of Forestry Sciences,Hohhot 010010,China;Forestry Station of Zhenglan Banner Forestry and Grassland Bureau,Inner Mongolia,Zhenglan Banner 027200,China;College of Forestry,Inner Mongolia Agricultural University,Hohhot 010019,China;Science and Technology Department,Inner Mongolia Normal University,Hohhot 010022,China)

机构地区：[1]内蒙古农业大学草原与资源环境学院,内蒙古呼和浩特010011 [2]植物学国家级实验教学示范中心,内蒙古呼和浩特010019 [3]内蒙古自治区土壤质量与养分资源重点实验室,内蒙古呼和浩特010011 [4]农业生态安全与绿色发展自治区高等学校重点实验室,内蒙古呼和浩特010011 [5]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010 [6]正蓝旗林业和草原局林业工作站,内蒙古正蓝旗027200 [7]内蒙古农业大学林学院,呼和浩特010019 [8]内蒙古师范大学科技处,内蒙古呼和浩特010022

出　　处：《内蒙古林业科技》2024年第2期29-34,共6页Journal of Inner Mongolia Forestry Science and Technology

基　　金：国家自然科学基金项目(32360422);内蒙古自治区自然科学基金项目(2021BS03023,2022LHQN03007);内蒙古自治区直属高校基本科研业务费项目(BR230163);内蒙古自治区高等学校科学技术研究项目(NJZY21462);内蒙古农业大学高层次人才引进科研启动项目(NDYB2019-22)。

摘　　要：渗透调节物质与抗氧化酶活性在植物生态适应中发挥着重要的调控作用。本研究对浑善达克沙地不同生境黄柳(Salix gordejevii)叶片渗透调节物质、抗氧化酶活性及丙二醛(MDA)含量进行测定,并分析其与土壤理化因子的相关性,以期揭示黄柳的生理生态适应机制。结果表明:(1)不同生境黄柳叶片游离脯氨酸(Pro)含量具有显著性差异,流动沙地Pro含量最大,为20.27μg.g^(-1),半固定沙地最小,为11.21μg.g^(-1);可溶性糖和可溶性蛋白含量无显著性差异。(2)不同生境黄柳叶片超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性具有显著性差异,丘间低地SOD活性(242.61 U.g^(-1))和CAT活性(337.50 U.g^(-1))高于其他生境;各生境过氧化物歧化酶(POD)活性和MDA含量无显著性差异。(3)不同生境间具有明显的异质性,但土壤理化因子与黄柳叶片渗透调节物质、抗氧化酶活性及MDA间无相关关系。Pro、SOD和CAT是黄柳适应浑善达克沙地不同生境的主要调节物质,反映了黄柳具有较强的生理生态适应性。Osmoregulatory substances and antioxidant enzymes activities play an important role in plant ecological adaptation.To reveal the physiological and ecological adaptation mechanism of Salix gordejevii,the osmoregulatory substances content,antioxidant enzymes activities,malondialdehyde(MDA)content of those leaves in different habitats in Otindag Sandy Land were measured,and the correlations between them and edaphic physicochemical factors were explored.The results showed as follows,(1)There was significant difference in proline(Pro)content among different habitats.The highest Pro content was 20.27μg.g^(-1)in mobile dune,and the lowest was 11.21μg.g^(-1)in semi-fixed dune.(2)Superoxide dismutase(SOD)and catalase(CAT)activities were significantly different in different habitats.SOD activities(242.61 U.g^(-1))and CAT activities(337.50 U.g^(-1))were significantly higher in dune slack than in other habitats.There were no significant differences in POD activities and MDA content among different habitats.(3)There was obvious heterogeneity among different habitats,but there were no correlation between soil physicochemical factors and osmoticregulatory substances,antioxidant enzyme activities and malondialdehyde content.It followed then Pro,SOD and CAT were the main regulatory substances for Salix gordejevii to adapt to different habitats in Otindag Sandy Land,which reflected the strong physiological and ecological adaptability of it.The results provided theoretical references for the study of ecological and biological characteristics of Salix gordejevii and its rational exploitation and utilization.

关 键 词：黄柳 渗透调节物质 抗氧化酶活性 生理生态适应 

分 类 号：S793.9[农业科学—林木遗传育种]