3株内生真菌对沙柳溃疡病病原菌的拮抗作用研究  

Antagonistic Effect of Three Endophytic Fungi on Canker Pathogen of Salix psammophila

作　　者：兰佳贺 姜海燕[1] 吴云菊 党海龙 蒋萌萌 房钰欣 LAN Jiahe;JIANG Haiyan;WU Yunju;DANG Hailong;JIANG Mengmeng;FANG Yuxin(College of Forestry,Inner Mongolia Agricultural University,Hohhot 010019,China;Yuquan Branch of Hohhot Forestry and Grassland Bureau,Inner Mongolia,Hohhot 010040,China;Saihan Branch of Hohhot Forestry and Grassland Bureau,Inner Mongolia,Hohhot 010010,China)

机构地区：[1]内蒙古农业大学林学院,内蒙古呼和浩特010019 [2]呼和浩特市林业和草原局玉泉分局,内蒙古呼和浩特010040 [3]呼和浩特市林业和草原局赛罕分局,内蒙古呼和浩特010010

出　　处：《内蒙古林业科技》2024年第2期35-39,共5页Journal of Inner Mongolia Forestry Science and Technology

基　　金：满洲里市草原有害生物及林业湿地外来有害生物普查采购项目(MZL-QY-22015);呼和浩特市草原有害生物及森林、草原、湿地外来入侵物种普查技术服务项目(FHYXFW2023-03001);内蒙古大学生创新创业项目(202210129029);内蒙古自治区2022年科学技术协会科普作品资助项目。

摘　　要：为探究沙地云杉(Picea meyeri var. mongolica)内生真菌(Endophytic fungi)对沙柳溃疡病病原菌茶藨子葡萄座腔菌(Botryosphaeria ribis)的抑菌作用,本研究选择互隔链格孢菌(Alternaria alternata)、三线镰刀菌(Fusarium tricinctum)、产黄青霉菌(Penicillium chrysogenum) 3种沙地云杉内生真菌作为试验材料,以沙柳溃疡病病原菌茶藨子葡萄座腔菌为目标菌,采用平板对峙法、生长速率法、双皿对扣法探究内生真菌对沙柳溃疡病病原菌的抑菌活性。结果表明:3株内生真菌均表现出良好的抑菌效果,菌株SDYS95产黄青霉菌的抑菌作用最好,抑菌率可达81.32%,其发酵液抑菌率可达22.29%,挥发性代谢产物抑菌率可达16.39%。为利用内生真菌及其代谢产物替代化学农药,保护北沙柳(Salix psammophila)资源提供参考。In order to explore the antibacterial effect of endophytic bacteria of Picea meyeri var.mongolica on the pathogen Botryosphaeria ribis,in this research,three endophytic fungi of Picea meyeri var.mongolica,namely Alternaria alternata,Fusarium tricinctum and Penicillium chrysogenum,were selected as the experimental materials,taking Botryosphaeria ribis,the antibacterial activity of endophytic fungi against the pathogenic bacteria of Botryosphaeria ribis,was studied by using plate method,growth rate method,and fermentation broth culture method.The results showed that all three endophytic strains showed good bacteriostatic effects,the bacterium SDYS95(Penicillium chrysogenum)had the best antibacterial activity,and the bacteriostatic rate was up to 81.32%,the bacteriostatic rate of fermentation fluid was up to 22.29%,and the bacteriostatic rate of volatile metabolites was up to 16.39%.It provided references for using endophytic fungi and their metabolites to replace chemical pesticides and protect the resource of Salix psammophila.

关 键 词：沙地云杉 内生真菌 沙柳溃疡病 拮抗作用 生物防治 

分 类 号：Q949.32[生物学—植物学]