HPLC多指标成分定量结合化学计量学、加权TOPSIS模型评价不同产地肺形草质量  

Evaluation of Tripterospermum chinense from different origins by HPLC multi index component combined with chemometrics and weighted TOPSIS model

作　　者：张立群[1] 马诗瑜[1] 冯恺 徐虹 ZHANG Liqun;MA Shiyu;FENG Kai;XU Hong(Department of Pharmacy,Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海交通大学医学院附属瑞金医院药剂科,上海200025 [2]上海中医药大学,上海200032

出　　处：《现代药物与临床》2024年第5期1176-1183,共8页Drugs & Clinic

基　　金：上海市“医苑新星”青年医学人才培养资助计划项目(沪卫人事[2022]65号)。

摘　　要：目的评价不同产地肺形草的质量差异。方法采用HPLC法测定肺形草中马钱苷酸、莫诺苷、獐牙菜苦苷、龙胆苦苷、芒果苷、异荭草苷、异牡荆素、去甲基雏菊叶龙胆酮、当药黄素、3,6,8-三羟基-1-甲基呫吨酮、山楂酸和熊果酸,对多指标成分测定结果进行主成分分析、正交偏最小二乘法-判别分析,建立加权优劣解距离模型评估。结果16批肺形草明显聚集为3类;马钱苷酸、莫诺苷、3,6,8-三羟基-1-甲基呫吨酮、熊果酸、当药黄素和芒果苷是影响肺形草质量的主要潜在标志物;浙江和江苏产肺形草整体质量较好。结论建立的方法控制模式简单、评价模型科学合理,可用于肺形草质量评价。Objective To evaluate the quality difference of Tripterospermum chinense from different origins.Methods Contents of loganic acid,morroniside,swertiamain,gentiopicrin,mangiferin,isoorientin,isovitexin,demethylbellidifolin,swertisin,3,6,8-trihydroxy-1-methyl-xanthen-9-one,maslinic acid and ursolic acid in T.chinense were determined by HPLC method.PCA and OPLSDA were used to analyze the contents of multi-component components,and the weighted-TOPSIS model was established to evaluate the quality.Results 16 Batches of T.chinense were clustered into three categories,and loganic acid,morroniside,3,6,8-trihydroxy1-methyl-xanthen-9-one,ursolic acid,swertisin,and mangiferin were the main potential markers affecting the quality of T.chinense.The overall quality of T.chinense from Zhejiang and Jiangsu Provinces is good.Conclusion The established method has a simple control mode and a scientifically reasonable evaluation model,which can be used for the quality evaluation of T.chinense.

关 键 词：肺形草 钱苷酸 莫诺苷 獐牙菜苦苷 龙胆苦苷 芒果苷 异荭草苷 异牡荆素 去甲基雏菊叶龙胆酮 当药黄素 3 6 8-三羟基-1-甲基呫吨酮 山楂酸 熊果酸 高效液相色谱 主成分分析 正交偏最小二乘法-判别分析 加权优劣解距离模型 

分 类 号：R286.02[医药卫生—中药学]