华山松切梢小蠹伴生真菌新种Geosmithia armandii及其对华山松的致病性  

Geosmithia armandii,a new fungal species associated with Tomicus armandii and its pathogenicity to Pinus armandii

作　　者：梁玲瑜 王慧敏 刘福 吕全[1] LIANG Lingyu;WANG Huimin;LIU Fu;LÜQuan(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Ecology and Nature Conservation Institute,Chinese Academy of Forestry,Beijing 100091,China)

机构地区：[1]中国林业科学研究院森林生态环境与自然保护研究所、国家林业和草原局森林保护学重点实验室,北京100091

出　　处：《菌物学报》2024年第6期14-28,共15页Mycosystema

基　　金：国家自然科学基金(32201569,32230071,32071769)。

摘　　要：Geosmithia(子囊菌门,肉座菌目)是常见的小蠹虫伴生真菌,其中部分类群能够入侵寄主并引起严重的植物病害。本研究通过真菌的分离培养,鉴定中国西南地区华山松切梢小蠹Tomicus armandii伴生真菌新种,并通过有伤接种实验对其致病性进行评估。对来自云南省玉溪市和富源县的45只华山松切梢小蠹成虫及64个坑道进行分离,获得387株Geosmithia属菌株,选取10株代表性菌株比较形态、生理特征并进行系统发育(ITS、TUB2和TEF1-α序列片段)分析。其中90个菌株用于构建ITS树,66个菌株用于TUB树,TEF树包含77个菌株。研究发现387株菌株代表1个新种,命名为Geosmithia armandii,G.armandii是第一个报道与华山松切梢小蠹伴生的Geosmithia属真菌。选取两株菌株接种至3年生华山松幼苗进行致病性测定,接种1个月后发现处理组接种处坏死病斑长度与对照组相似,结果表明该新种为弱致病菌。本研究为今后Geosmithia与华山松切梢小蠹伴生关系的研究奠定基础,为防治华山松切梢小蠹提供新思路。Species of Geosmithia(Ascomycota,Hypocreales)are commonly associated with bark beetles,and several species can infect tree hosts and cause serious diseases.In this study,a culture-dependent and pathogenic trial was conducted to classify a species of Geosmithia associated with Tomicus armandii infesting Pinus armandii in southwest China.In total,387 Geosmithia isolates were obtained from 45 adults and 64 galleries of T.armandii in Yuxi and Fuyuan Cities,Yunnan Province,China.Ten representative isolates were further identified on the basis of morphological and physiological characteristics and the phylogenetic analyses were conducted based on ITS,TUB2,and TEF1-αsequences.Among these,90 isolates were used for the establishment of the ITS tree,66 of the TUB tree,and 77 of the TEF tree.As a result,a novel species,G.armandii was identified.This is the first species of Geosmithia reported to be associated with T.armandii.In vivo pathogenicity analysis of the two isolates selected indicated that in one month after inoculation,there was no significant difference between average lesion length caused by the inoculated isolates and the experimental control,showing that G.armandii is a weak pathogen of Pinus armandii.

关 键 词：Geosmithia 形态学 系统学分析 致病性 新种 

分 类 号：S763.7[农业科学—森林保护学]