利用转录组初步探索曲古抑素A延缓香菇菌丝转色机制  

Transcriptome was used to preliminarily explore the mechanism of trichostatin A delaying the formation of brown mycelium membrane of Lentinula edodes

作　　者：吴涛 鲍大鹏[2] 王伟霞 仝乐涛 李福后 唐利华[2] WU Tao;BAO Dapeng;WANG Weixia;TONG Letao;LI Fuhou;TANG Lihua(School of Ocean Food and Biological Engineering,Jiangsu Ocean University,Lianyungang 222005,Jiangsu,China;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]江苏海洋大学海洋食品与生物工程学院,江苏连云港222005 [2]上海市农业科学院食用菌研究所,上海201403

出　　处：《菌物学报》2024年第6期59-72,共14页Mycosystema

基　　金：国家自然科学基金(32172648)。

摘　　要：组蛋白去乙酰化是最重要的表观遗传修饰之一,在发育中起着多种作用,但组蛋白去乙酰化在香菇中的详细功能和机制尚不清楚。本研究使用广泛的去乙酰化抑制剂(HDACi)曲古抑素A(TSA)来确定组蛋白去乙酰化对香菇发育过程的作用。通过香菇菌包的培养,观察到外源性应用TSA能够抑制香菇后熟转色的进程。随后,我们进行了转录组学分析以揭示潜在的机制。数据显示,TSA处理产生393个差异表达基因,这些基因主要富集于次生代谢物的生物合成、内质网中的蛋白质加工、不同环境中的微生物代谢等途径。通过分析TSA处理和对照组的基因表达差异,发现TSA可能通过影响组蛋白乙酰化水平直接或间接影响细胞色素P450、漆酶,糖蛋白家族等相关基因,从而影响香菇菌丝转色。为认识组蛋白去乙酰化对香菇后熟转色发育的影响提供依据,进一步为香菇工厂化生产缩短周期提供一定的借鉴作用。Histone deacetylation is one of the most important epigenetic modifications and plays a variety of roles in development,but the detailed function and mechanism of histone deacetylation in Lentinula edodes is unknown.An extensive deacetylation inhibitor(HDACi),trichostatin A(TSA)was used to determine the role of histone deacetylation on the developmental process of Lentinula edodes.It was observed that exogenous application of TSA could inhibit the process of ripening and brown mycelium film formation of pack-cultured Lentinula edodes.Transcriptomic analysis revealed that TSA treatment produced 393 differentially expressed genes,which were mainly enriched in the biosynthesis of secondary metabolites,protein processing in the endoplasmic reticulum,and microbial metabolism in different environments.Analysis of the differences in gene expression between TSA treatment and control groups indicated that TSA might directly or indirectly affect the genes of cytochrome P450,laccase,and glycoprotein family by affecting histone acetylation levels,and thereby exert an influence on the formation of Lentinula edodes brown mycelium membrane.This paper provides a reference helpful to shortening the cycle of industrial production of Lentinula edodes.

关 键 词：转录组 组蛋白去乙酰化 香菇转色 曲古抑素A 

分 类 号：S646.12[农业科学—蔬菜学]