清远麻鸡miR-142-3p分析及其关键靶基因的筛选  

MiR-142-3p analysis of Qingyuan partridge chickens and screening of key target genes

作　　者：朱师良 余春林[1] 邱莫寒[1] 张增荣[1] 熊霞[1] 胡陈明[1] 杨礼[1] 彭涵[1] 陈家磊 宋小燕[1] 夏波[1] 杨朝武[1] ZHU Shiliang;YU Chunlin;QIU Mohan;ZHANG Zengrong;XIONG Xia;HU Chenming;YANG Li;PENG Han;CHEN Jialei;SONG Xiaoyan;XIA Bo;YANG Chaowu(Animal Breeding and Genetics Key Laboratory,Sichuan Animal Science Academy,Chengdu 610066,China)

机构地区：[1]四川省畜牧科学研究院动物遗传育种重点实验室,成都610066

出　　处：《中国农业大学学报》2024年第7期12-22,共11页Journal of China Agricultural University

基　　金：国家肉鸡产业技术体系项目(CARS-41-G07);“十四五”四川省畜禽育种攻关项目(2021YFYZ0031);四川省区域创新合作项目(2023YFQ0035)。

摘　　要：为分析清远麻鸡不同组织中miR-142-3p的表达情况,并筛选其在脾脏中的关键靶基因,本研究利用在线数据库预测鸡miR-142-3p靶基因,并对靶基因进行GO和KEGG富集分析以及构建蛋白-蛋白互作网络。利用在线公共数据库,分析脾脏相对于其他组织的miR-142-3p表达量;再根据miRNA表达量与其靶基因表达量负相关机制,对miR-142-3p靶基因与脾脏中相对其他组织显著下调基因取交集,筛选潜在靶基因。结果表明:1)预测获得85个靶基因,GO富集分析显示,靶基因参与多种高分子化合物的合成与转运过程。2)KEGG结果显示富集到MAPK、Notch、Wnt等多条与抗肿瘤免疫相关的信号通路。3)PPI网络共筛选到4个Hub基因。4)miR-142-3p在脾脏中的表达量极显著高于肝脏、肺脏、胸肌、心脏和肾脏(P<0.01)。5)共筛选到TWF1、MYH10、NFIB、FNBP1L和FERMT25个靶基因。综上,鸡miR-142-3p在脾脏中很可能通过TWF1、MYH10、NFIB、FNBP1L和FERMT2发挥生物学功能。本研究为解析鸡miR-142-3p的功能及其分子机制提供了参考和依据。The aim of the study is to analyze the expression of miR-142-3p in different tissues of Qingyuan partridge chickens and screen for its key target genes in spleen.In this study,the online database was used to predict the target genes of chicken miR-142-3p,and the GO,KEGG and protein-protein interaction network analysis of the target genes were performed.The online public database was used to analyze the expression of miR-142-3p in spleen compared with other tissues.Based on the mechanism of negative correlation between miRNA expression and target gene expression,the intersection of miR-142-3p target genes and significantly down-regulated genes in spleen compared to other tissues was taken to screen potential target genes.The results showed that:1)A total of 85 target genes were obtained,and GO enrichment analysis showed that the target genes were involved in the synthesis and transport of a variety of polymers.2)KEGG results showed that MAPK,Notch,Wnt and other signaling pathways related to anti-tumor immunity were enriched.3)A total of 4 Hub genes were screened by PPI network.4)miR-142-3p expression was significantly higher in spleen than that in liver,lung,pectoral muscle,heart and kidney.5)Five target genes were screened including TWF1、MYH10、NFIB、FNBP1L and FERMT2.In summary,chicken miR-142-3p is likely to exert biological functions in the spleen through TWF1、MYH10、NFIB、FNBP1L and FERMT2.This study provides a reference and basis for analyzing the function and molecular mechanism of chicken miR-142-3p.

关 键 词：清远麻鸡 miR-142-3p 生物信息学 组织表达 脾脏 

分 类 号：S831.2[农业科学—畜牧学]