Status and developmental trends in recombinant collagen preparation technology  

作　　者：Xiaolei Guo Yuan Ma Hang Wang Hongping Yin Xinli Shi Yiqin Chen Guobiao Gao Lei Sun Jiadao Wang Yunbing Wang Daidi Fan 

机构地区：[1]Center for Medical Device Evaluation,National Medical Products Administration,Beijing 100081,China [2]State Key Laboratory of Tribology,Tsinghua University,Beijing 100084,China [3]school of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China [4]National Engineering Research Center for Biomaterials&College of Biomedical Engineering,Sichuan University,Chengdu 610064,China [5]Biotech.&Biomed.Research Institute,Northwest University,Xi'an 710127,China

出　　处：《Regenerative Biomaterials》2024年第2期17-36,共20页再生生物材料（英文版）

基　　金：supported by the second batch of the China Drug Regulatory Science Action Plan(Research on safety and effectiveness evaluation of novel biomaterials)from National Medical Products Administration(NMPA).

摘　　要：Recombinant collagen is a pivotal topic in foundational biological research and epitomizes the application of critical bioengineer-ing technologies.These technological advancements have pro-found implications across diverse areas such as regenerative medicine,organ replacement,tissue engineering,cosmetics and more.Thus,recombinant collagen and its preparation methodologies rooted in genetically engineered celis mark pivotal milestones in medical product research.This article pro-vides a comprehensive overview of the current genetic engi-neering technologies and methods used in the production of recombinant collagen,as well as the conventional production process and gquality control detection methods for this material.Furthermore,the discussion extends to foresee the strides in physical transfection and magnetic control sorting studies,envisioning an enhanced preparation of recombinant collagen-seeded cells to further fuel recombinant collagen production.

关 键 词：recombinant collagen preparation genetic engineering collagen characterization physical transfection seed cell sorting 

分 类 号：R318[医药卫生—生物医学工程]