水痘-带状疱疹病毒Oka-7S株的免疫原性分析  

Immunogenicity study of varicella-zoster virus Oka-7S strain

作　　者：张树杰 戴龙 范滢 周宏达 房佳 王梦涵 徐俊峰 张夫坤 ZHANG Shujie;DAI Long;FAN Ying;ZHOU Hongda;FANG Jia;WANG Menghan;XU Junfeng;ZHANG Fukun(Changchun Keygen Biological Products Co.,Ltd.,Changchun 130000,Jilin Province,China)

机构地区：[1]长春祈健生物制品有限公司,吉林长春130000

出　　处：《中国生物制品学杂志》2024年第6期660-665,共6页Chinese Journal of Biologicals

基　　金：吉林省与中国科学院科技合作高新技术产业化专项资金(2020SYHZ0010)。

摘　　要：目的比较水痘-带状疱疹病毒(varicella-zoster virus,VZV)Oka-7S与Oka株免疫原性。方法用高、中、低(1000、250、62.5 PFU/剂)剂量VZV Oka-7S及Oka株皮下分别免疫雌性BALB/c小鼠,每组12只,共免疫2次,每次间隔14 d。基于膜抗原荧光抗体试验(fluorescent antibody to membrane antigen assay,FAMA)进行VZV特异性体液免疫评价,酶联免疫斑点(ELISPOT)试验进行VZV特异性细胞免疫评价。结果VZV Oka-7S和VZV Oka株均可引起特异性体液免疫应答和细胞免疫应答,且具有相当的免疫效果;随免疫次数增加和免疫剂量升高,Oka-7S-2.2组小鼠血清中抗VZV特异性IgG抗体水平均明显升高。结论VZV Oka-7S株免疫原性与VZV Oka株相当。Objective To compare the immunogenicity of varicella-zoster virus(VZV)Oka-7S strain and VZV Oka strain.Methods Female BALB/c mice were subcutaneously immunized with VZV Oka-7S and Oka strains at high,medium and low doses(1000,250 and 62.5 PFU/dose)for total two doses at an interval of 14 d,separately,with 12 mice in each group.The VZV-specific humoral immunity was evaluated based on fluorescent antibody to membrane antigen assay(FAMA),and the VZV-specific cellular immunity was evaluated by enzyme-linked immunospot assay(ELISPOT).Results Both VZV Oka-7S and VZV Oka strains induced specific humoral and cellular immune responses,and showed considerable immune effects;The level of anti-VZV specific IgG in serum of mice in Oka-7S-2.2 group increased significantly with the increase of immunization times and immunization doses.Conclusion The VZV Oka-7S strain had comparative immunogenicity with VZV Oka strain.

关 键 词：水痘-带状疱疹病毒 酶联免疫斑点试验 膜抗原荧光抗体试验 免疫原性 

分 类 号：R373.12[医药卫生—病原生物学]