山东湿地生防菌筛选及解淀粉芽孢杆菌JQ-3的防病促生作用  

Screening of biocontrol bacteria from wetland of Shandong and the disease control and growth promotion effect of Bacillus amyloliquefaciens JQ-3

作　　者：张淑静[1] 张淑凤 杨萍 肖琳[4] 曲田丽[4] 张保华[4] 牛赡光[1] 杜丰玉[4] Zhang Shujing;Zhang Shufeng;Yang Ping;Xiao Lin;Qu Tianli;Zhang Baohua;Niu Shanguang;Du Fengyu(Institute of Forest Protection and Biological Drugs,Shandong Academy of Forest Sciences,Ji’nan Shandong 250014,China;Qingdao Nobel Bioengineering Co.Ltd,Qingdao Shandong 266600,China;Laixi Reservoir Management and Service Center,Qingdao Shandong 266600,China;College of Chemistry and Pharmacy,Qingdao Agricultural University,Qingdao Shandong 266109,China)

机构地区：[1]山东省林业科学研究院森林保护与生物药物研究所,山东济南250014 [2]青岛秾贝尔生物工程有限公司,山东青岛266600 [3]山东省莱西市水库管理服务中心,山东青岛266600 [4]青岛农业大学化学与药学院,山东青岛266109

出　　处：《中国植保导刊》2024年第5期16-21,共6页China Plant Protection

基　　金：山东省科技型中小企业创新能力提升工程项目(2021TSGC1249);青岛市科技惠民示范引导专项(22-3-7-xdny-6-nsh)。

摘　　要：山东为湿地大省,为充分发掘湿地微生物所蕴藏的生防潜力,以耐药灰霉菌为靶标,从山东省内14处湿地累计分离到具拮抗作用的生防菌143株,其中拮抗作用较强的包括5株解淀粉芽孢杆菌、4株枯草芽孢杆菌以及较为少见的沙福芽孢杆菌、南海芽孢杆菌各1株。选择对灰霉菌菌丝生长抑制力最强的解淀粉芽孢杆菌JQ-3,初步调查其防病促生作用,发现该菌能较好地预防葡萄果实感染灰霉病,在花生幼苗盆栽试验中表现出对白绢病的预防作用,并能促进幼苗根系生长。综上得出,山东湿地蕴藏着较丰富的生防微生物资源,本研究分离得到的解淀粉芽孢杆菌JQ-3可用于功能菌剂的开发。The aim of this study was to investigate the potential of biocontrol bacteria from wetlands in Shandong province.143 biocontrol strains against Botrytis cinerea were isolated from 14 distinct wetlands in Shandong province.Among the strains identified,five were of Bacillus amyloliquefaciens,four were Bacillus subtilis,one was Bacillus safensis and one was Bacillus nanhaiensis.These strains exhibited a significant antagonistic effect on Botrytis cinerea.Among the strains,Bacillus amyloliquefaciens JQ-3 showed the most pronounced inhibitory effect on the growth of Botrytis cinerea,demonstrating the potential to prevent grape from being infected with Botrytis cinerea.In the pot experiment,it showed an evident control effect against Sclerotium rolfsii,while also promoting root growth of peanut seedlings.In conclusion,the wetlands in Shandong province represents a promising source of biocontrol microbial resources,and Bacillus amyloliquefaciens JQ-3 could be further developed into a functional bacterial fertilizer.

关 键 词：山东湿地 农用生防菌 灰霉病 花生白绢病 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]