烯虫酯对脱果后桃小食心虫幼虫发育的干扰作用  

Effect of methoprene on the development of Carposina sasakii mature larvae

作　　者：李永丽 周洲 闫作炳 乔利 张方梅 Li Yongli;Zhou Zhou;Yan Zuobing;Qiao Li;Zhang Fangmei(College of Agriculture,Xinyang Agriculture and Forestry University/Xinyang Key Laboratory of Agricultural Microbial Resources Development and Utilization,Xinyang,Henan 464000,China;Xinyang Plant Protection Microorganism Engineering Technology Center,Henan Province,Xinyang,Henan 464000)

机构地区：[1]信阳农林学院农学院/信阳市农业微生物资源开发与利用重点实验室,河南信阳464000 [2]河南省信阳市植保微生物工程技术中心,河南信阳464000

出　　处：《中国植保导刊》2024年第5期62-64,76,共4页China Plant Protection

基　　金：国家自然科学基金资助项目(31870638);信阳农林学院高水平科研孵化器建设基金项目(FCL202109);信阳农林学院作物绿色防控与品质调控科技创新团队建设项目(XNKJTD-007);信阳市重点研发与推广专项项目(20220061);河南省高等学校重点科研项目(23A210028)。

摘　　要：为探究烯虫酯对桃小食心虫发育的干扰作用,开展了室内试验,采用药剂点滴法和喷砂法处理短光照条件下的脱果幼虫后,发现试虫结茧异常占比约为30%,结茧异常比例不随药剂浓度升高而增加;烯虫酯1mg/L喷沙组最终解除滞育的试虫中仅有12.5%能够羽化,更高浓度处理组试虫均不能羽化。采用药剂点滴法处理长光照条件下的脱果幼虫后,结茧异常占比约为25%,蛹内发育受到明显影响,许多试虫初期正常结长茧,但后来在茧内死亡,死亡率随药剂浓度的升高而升高,浓度50 mg/L以上处理的试虫死亡率为100%;药剂喷沙处理中,烯虫酯浓度1 mg/L和10 mg/L喷沙组的试虫羽化率分别为40%和10%,更高浓度组的试虫均在长茧内死亡,没有羽化。通过明确烯虫酯对脱果幼虫发育的干扰作用,为地面喷施该药防治脱果桃小食心虫幼虫提供了依据。This study was aim to develop a new technology for the control of Carposina sasakii.The mature larvae of C.sasakii,which exited from the fruits in response to short-or long-light stimulation,were treated with methoprene by the drip method and sandblast method.The results showed that the proportion of cocooning abnormalities,including non-cocooning,abnormal cocooning,and long cocoon,was approximately 30%.Moreover,the proportion of cocooning abnormalities did not increase with the increase in methoprene concentration.Only 12.5%of the larvae with compressed cocoons were able to emerge from diapause in the 1 mg/L sandblast group,while none of the larvae were able to emerge in the treatment with higher methoprene concentration.The proportion of abnormal cocooning in the mature larvae of C.sasakii under long-light treatment by drip method was 25%approximately.The phenotypes of abnormal cocooning were irregular,and the development in pupae was significantly affected.Many of the insects tested initially had normal cocooning,but subsequently died in the cocoons.The mortality rate increased with the increase in methoprene concentration,with a mortality rate of 100%ovserved at concentrations above 50 mg/L.In the sandblast treatment,the eclosion rates of C.sasakii larvae in the 1 mg/L and 10 mg/L sandblast groups were 40%and 10%,respectively.The higher concentration groups died in the long cocoons without eclosion.Methoprene is environmentally friendly and harmless to humans and animals.This study clarified the developmental disruption effect of methoprene on the subsequent development of mature larvae of C.sasakii,therby providing a valuable reference for future control of C.sasakii.

关 键 词：桃小食心虫 烯虫酯 干扰发育 化学防治 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]