Colorimetric Detection of Acid Phosphatase and Malathion Using NiCo_(2)O_(4)Nanosheets as Peroxidase-Mimicking Activity  

作　　者：Jia Chen Zerong Long Yan Li Hongdeng Qiu 

机构地区：[1]Xinjiang Uygur Autonomous Product Quality Supervision and Inspection Institute,Urumqi,830000,China [2]CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou,730000,China

出　　处：《Journal of Analysis and Testing》2024年第2期170-179,共10页分析检测（英文）

基　　金：financially supported by the Regional Collaborative Innovation Project of Xinjiang(2020E01051);the Youth Innovation Promotion Association CAS(2021420)。

摘　　要：The establishment of a low-cost and facile approach for the assay of acid phosphatase(ACP)and its inhibitor is a research focus.In this paper,we successfully synthesized NiCo_(2)O_(4)nanosheets with peroxidase-mimicking activity in alkaline deep eutectic solvents(DESs)composed of NaOH and PEG-200,which can catalyze 3,3',5,5'-tetramethylbenzidine(TMB)to the oxidation state TMB(ox-TMB)in the presence of H2O2.Due to L-ascorbic acid-2-phosphate can be hydrolyzed into ascorbic acid by ACP,the peroxidase-like activity of NiCo_(2)O_(4)nanosheets is inhibited.However,the addition of malathion,an inhibitor of ACP,can inhibit the activity of ACP and thus limit the production of ascorbic acid(AA).Therefore,a colorimetric approach can be established for highly sensitive and selective detection of ACP and malathion with detection limits of 0.032 U/L and 0.65 nmol/L,respectively.More importantly,this method can be successfully applied to malathion detection in environmental samples tap water and food cucumber juice,indicating that it has potential application prospects in environmental and agricultural fields.

关 键 词：NiCo_(2)O_(4)nanosheets Acid phosphatase MALATHION Peroxidase-mimicking activity COLORIMETRIC 

分 类 号：X832[环境科学与工程—环境工程] TS255.44[轻工技术与工程—农产品加工及贮藏工程] O657.3[轻工技术与工程—食品科学与工程]