Targeting Haemaphysalis longicornis serpin to prevent tick feeding and pathogen transmission  

作　　者：Tingting Feng Hao Tong Qianqian Zhang Zhihao Ming Zhenyu Song Xia Zhou Jianfeng Dai 

机构地区：[1]Institute of Biology and Medical Sciences,Jiangsu Key Laboratory of Infection and Immunity,Soochow University,Suzhou,Jiangsu Province,China [2]School of Biology and Basic Medical Science,Suzhou Medical College of Soochow University,Suzhou,Jiangsu Province,China

出　　处：《Insect Science》2024年第3期694-706,共13页昆虫科学（英文版）

基　　金：supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions,National Natural Science Foundation of China(81971917,32170142,81271792,and 81471571);Jiangsu Natural Science Foundation(BK20211310).

摘　　要：Serine proteinase inhibitors(serpins),identified from the hard tick Haemaphysalis longicornis of China,play significant roles in various animal physiological processes.In this study,we showed that H.longicornis serpins(Hlserpin-a and Hlserpin-b)were induced during blood-feeding in nymph ticks and exhibited anticoagulation activity in vitro.Silencing Hlserpins through RNA interference(RNAi)significantly impaired tick feeding.Immunization of mice with recombinant Hlserpins or passive transfer of Hlserpin antiserum significantly curtails the efficacy of tick feeding.Concurrently,the transmission of the Langat virus(LGTV)from ticks to mice witnessed a substantial decrease when Hlserpins were silenced.Our findings suggest that inhibiting Hlserpins can hamper tick engorgement and pathogen transmission,indicating the potential of Hlserpins as a vaccine to countertick-borne diseases.

关 键 词：FEEDING LGTV SERPIN TBEV TICK 

分 类 号：Q96[生物学—昆虫学]