Role of aminopeptidase N-like in the acquisition of begomoviruses by Bemisia tabaci,the whitefly vector  

作　　者：Yun-Yun Fan Yao Chi Na Chen Wilmer J.Cuellar Xiao-Wei Wang 

机构地区：[1]Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Insect Sciences,Zhejiang University,Hangzhou,China [2]Zhejiang Tianmushan National Nature Reserve Administration,Hangzhou,China [3]Institute of Plant Protection,Liaoning Academy of Agricultural Sciences,Shenyang,China [4]Virology Laboratory,Cassava Program,International Center for Tropical Agriculture(CIAT),Palmira,Colombia

出　　处：《Insect Science》2024年第3期707-719,共13页昆虫科学（英文版）

基　　金：Financial support for this article was provided by the National Natural Science Foundation of China(32161143008,31925233);the Bill&Melinda Gates Foundation(Investment ID OPP1149777);Wilmer J.Cuellar,is supported by the Australian Centre for International Agricultural Research(ACIAR),and the One CGIAR initiative on Plant Health(PHI).

摘　　要：Sri Lankan cassava mosaic virus(SLCMV)is a prominent causative agent of cassava mosaic disease in Asia and relies on the whitefly Bemisia tabaci cryptic complex for its transmission.However,the molecular mechanisms involved in SLCMV transmission by B.tabaci have yet to be understood.In this study,we identified an aminopeptidase N-like protein(BtAPN)in B.tabaci Asia II 1,an efficient vector of SLCMV,which is involved in the SLCMV transmission process.Through the use of glutathione S-transferase pull-down assay and LC-MS/MS analysis,we demonstrated the interaction between BtAPN and the coat protein(CP)of SLCMV.This interaction was further confirmed in vitro,and we observed an induction of BtAPN gene expression following SLCMV infection.By interfering with the function of BtAPN,the quantities of SLCMV were significantly reduced in various parts of B.tabaci Asia II 1,including the whole body,midgut,hemolymph,and primary salivary gland.Furthermore,we discovered that BtAPN is conserved in B.tabaci Middle East-Asia Minor 1(MEAM1)and interacts with the CP of tomato yellow leaf curl virus(TYLCV),a begomovirus known to cause severe damage to tomato production.Blocking BtAPN with antibody led to a significant reduction in the quantities of TYLCV in whitefly whole body and organs/tissues.These results demonstrate that BtAPN plays a generic role in interacting with the CP of begomoviruses and positively regulates their acquisition by the whitefly.

关 键 词：BtAPN coat protein SLCMV TYLCV virus acquisition WHITEFLY 

分 类 号：S43[农业科学—农业昆虫与害虫防治]