Cytoplasmic FKBPs are involved in molting and metamorphosis through regulating the nuclear localization of EcR  

作　　者：Xian Zhang Qiao-Ran Wang Qian Wu Jun Gu Li-Hua Huang 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]Guangmeiyuan R&D Center,Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou,China

出　　处：《Insect Science》2024年第3期759-772,共14页昆虫科学（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(grant no.32272523);the Natural Science Foundation of Guangdong Province,China(grant no.2023A1515010178);the Laboratory of Lingnan Modern Agriculture Project(grant no.NT2021003).

摘　　要：Molting and metamorphosis are important physiological processes in insects that are tightly controlled by ecdysone receptor(EcR)through the 20-hydroxyecdysone(20E)signaling pathway.EcR is a steroid nuclear receptor(SR).Several FK506-binding proteins(FKBPs)have been identified from the mammal SR complex,and are thought to be involved in the subcellular trafficking of SR.However,their roles in insects are poorly understood.To explore whether FKBPs are involved in insect molting or metamorphosis,we injected an FKBP inhibitor(FK506)into a lepidopteran insect,Spodoptera litura,and found that molting was inhibited in 61.11%of the larvae,and that the time for larvae to pupate was significantly extended.A total of 10 FKBP genes were identified from the genome of s.litura and were clustered into 2 distinct groups,according to their subcellular localization,with FKBP13 and FKBP14 belonging to the endoplasmic reticulum(ER)group and with the other members belonging to the cytoplasmic(Cy)group.All the CyFKBPs were significantly upregulated in the prepupal or pupal stages,with the opposite being observed for the ER group members.FK506 completely blocked the transfer of EcR to the nucleus under 20E induction,and significantly downregulated the transcriptional expression of many 20E signaling genes.A similar phenomenon was observed after RNA interference of2 CyFKBPs(FKBP45 and FKBP12b),but not for FKBP13.Taken together,our data indicate that the cytoplasmic FKBPs,especially FKBP45 and FKBP12b,mediate the nuclear localization of EcR,thereby regulating the 20E signaling and ultimately affecting molting and metamorphosis in insects.

关 键 词：ECR FKBP METAMORPHOSIS MOLTING RNAi subcellular localization 

分 类 号：Q96[生物学—昆虫学]