Mutations of voltage-gated sodium channel contribute to pyrethroid resistance in Panonychus citri  

作　　者：Deng Pan Qiu-Juan Luo Andrias O.O'Reilly Guo-Rui Yuan Jin-Jun Wang Wei Dou 

机构地区：[1]Key Laboratory of Entomology and Pest Control Engineering,College of Plant Protection,Academy of Agricultural Sciences,Southwest University,Chongqing,China [2]Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education),Academy of Agricultural Sciences,Southwest University,Chongqing,China [3]School of Biological and Environmental Sciences,Liverpool John Moores University,Liverpool,UK

出　　处：《Insect Science》2024年第3期803-816,共14页昆虫科学（英文版）

基　　金：supported by the Science and Technology Basic Resources Investigation Program of China(2018FY101105);the National Natural Science Foundation of China(31972272,31871969);the Fundamental Research Funds for the Central Universities(SWU-XDPY22001)of China;the China Agricultural Research System of MOA and MARA.

摘　　要：Insecticide resistance in Panonychus citri is a major obstacle to mite control in citrus orchards.Pyrethroid insecticides are continually used to control mites in China,although resistance to pyrethroids has evolved in some populations.Here,the resistance to the pyrethroid fenpropathrin was investigated and 7 out of 8 field-collected populations of P citri exhibited a high level of resistance,ranging from 171-fold to 15391-fold higher than the susceptible(SS)comparison strain.Three voltage-gated sodium channel(VGSC)mutations were identified in the tested populations:L1031V,F1747L,and F17511.Amplicon sequencing was used to evaluate the frequency of these mutations in the 19 field populations.L1031V and F1751I were present in all populations at frequencies of 11.6%-82.1%and 0.5%-31.8%,respectively,whereas the F1747L mutation was only present in 12 populations from Chongqing,Sichuan,Guangxi,and Yunnan provinces.Introduction of these mutations singly or in combination into transgenic flies significantly increased their resistance to fenpropathrin and these flies also exhibited reduced mortality after exposure to the pyrethroids permethrin andβ-cypermethrin.Panonychus citri VGSC homology modeling and ligand docking indicate that F1747 and F1751 form direct binding contacts with pyrethroids,which are lost with mutation,whereas L1031 mutation may diminish pyrethroid effects through an allosteric mechanism.Overall,the results provide molecular markers for monitoring pest resistance to pyrethroids and offer new insights into the basis of pyrethroid actions on sodium channels.

关 键 词：amplicon sequencing kdr mutation molecular docking Panonychus citri pyrethroid resistance transgenic flies 

分 类 号：Q96[生物学—昆虫学]