Risk assessment,fitness cost,cross-resistance,and mechanism of tetraniliprole resistance in the rice stem borer,Chilo suppressalis  

作　　者：Hao Sun Shuai Wang Chong Liu Wen-Kai Hu Jin-Wei Liu Ling-Jun Zheng Meng-Yue Gao Fang-Rui Guo Song-Tao Qiao Jun-Li Liu Bo Sun Cong-Fen Gao Shun-Fan Wu 

机构地区：[1]College of Plant Protection,Nanjing Agricultural University,State&Local Joint Engineering Research Center of Green Pesticide Invention and Application,Nanjing,China [2]Bayer Cropscience(China)Co.,Ltd.,Hangzhou,China

出　　处：《Insect Science》2024年第3期835-846,共12页昆虫科学（英文版）

基　　金：funded by the National Natural Science Foundation of China to SFW(32272576);the National Key Research and Developmental Program of China(2022YFD1400900).

摘　　要：The rice stem borer(RSB),Chilo suppressalis,a notorious rice pest in China,has evolved a high resistance level to commonly used insecticides.Tetraniliprole,a new anthranilic diamide insecticide,effectively controls multiple pests,including RSB.However,the potential resistance risk of RSB to tetraniliprole is still unknown.In this study,the tetraniliprole-selection(Tet-R)strain was obtained through 10 continuous generations of selection with tetraniliprole 30%lethal concentration(LC_(30)).The realized heritability(h^(2))of the Tet-R strain was 0.387,indicating that resistance of RSB to tetraniliprole developed rapidly under the continuous selection of tetraniliprole.The Tet-R strain had a high fitness cost(relative fitness=0.53).We established the susceptibility baseline of RSB to tetraniliprole(lethal concentration at LC_(50)=0.727 mg/L)and investigated the resistance level of 6 field populations to tetraniliprole.All tested strains that had resistance to chlorantraniliprole exhibited moderate-to high-level resistance to tetraniliprole(resistance ratio=27.7-806.8).Detection of ryanodine receptor(RyR)mutations showed that the Y4667C,Y4667D,I4758M,and Y4891F mutations were present in tested RSB field populations.RyR mutations were responsible for the cross-resistance between tetraniliprole and chlorantraniliprole.Further,the clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated protein 9-mediated genome-modified flies were used to study the contribution of RyR mutations to tetraniliprole resistance.The order of contribution of a single RyR mutation to tetraniliprole resistance was Y4667D>G4915E>Y4667C~~I4758M>Y4891F.In addition,the I4758M and Y4667C double mutations conferred higher tetraniliprole resistance than single Y4667C mutations.These results can guide resistance management practices for diamides in RSB and other arthropods.

关 键 词：Chilo suppressalis diamide resistance fitness cost CROSS-RESISTANCE RyR target-site mutations 

分 类 号：Q96[生物学—昆虫学]