经典名方五味消毒饮UPLC指纹图谱及关键质量属性量值传递规律研究  

UPLC fingerprint and quantity transfer law of key quality attributes of classic prescription Wuwei Xiaodu Yin

作　　者：张丽先 李晓[1] 李飞飞[1] 宁二娟 魏悦[1] 纵伟 ZHANG Li-xian;LI Xiao;LI Fei-fei;NING Er-juan;WEI Yue;ZONG Wei(Henan Academy of Sciences,Zhengzhou 450046,China;School of Food and Biological Engineering,Zhengzhou University of Light Industry,Zhengzhou 450066,China)

机构地区：[1]河南省科学院,郑州450046 [2]郑州轻工业大学食品与生物工程学院,郑州450066

出　　处：《中国新药杂志》2024年第12期1281-1288,共8页Chinese Journal of New Drugs

基　　金：河南省科技攻关项目(222102310021);河南省科学院基本科研费资助项目(230613040)。

摘　　要：目的:建立五味消毒饮UPLC指纹图谱,探究五味消毒饮物质基准制备过程中从中药饮片到物质基准之间关键质量属性量值传递关系。方法:水煎法制备15批五味消毒饮物质基准,并建立物质基准UPLC指纹图谱分析方法指认关键色谱峰,多波长同时测定绿原酸、秦皮乙素、菊苣酸、木犀草苷、3,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸和蒙花苷的含量,计算转移率和出膏率。结果:15批物质基准指纹图谱相似度>0.9,指认13个色谱峰,设定7个特征色谱峰。15批物质基准出膏率范围为28.72%~33.85%,绿原酸、秦皮乙素、菊苣酸、木犀草苷、3,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸和蒙花苷转移率范围分别为35.39%~42.48%,49.72%~61.20%,28.47%~34.30%,28.96%~39.20%,20.09%~23.57%,81.13%~95.02%,30.33%~35.92%,未出现离散数据。结论:研究五味消毒饮UPLC指纹图谱分析方法及其关键质量属性量值传递规律对于其物质基准的开发与应用具有参考价值。Objective:To establish the UPLC fingerprint of Wuwei Xiaodu Yin and explore the transfer relationship of key quality attributes from traditional Chinese medicine slices to the reference material.Methods:Fifteen batches of substance benchmarks were prepared by decoction method.The UPLC fingerprints of the substance benchmarks were detected,and the key peaks of fingerprints were identified.The contents of chlorogenic acid,esculetin,cichoric acid,luteolosidein,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid and acaciin were determined at multiple wavelengths simultaneously.The transfer rate and extract rate were calculated.Results:The similarities of the UPLC fingerprints of the substance benchmarks were over 0.9.Thirteen peaks were identified among which seven peaks were set as characteristic peaks.The extract rates of 15 batches of substance benchmarks ranged from 28.72%to 33.85%.The transfer rates of chlorogenic acid,esculetin,cichoric acid,luteolosidein,3,5-O-Dicaffeoylquinic acid,4,5-O-Dicaffeoylquinic acid and acaciin were 35.39%~42.48%,49.72%~61.20%,28.47%~34.30%,28.96%~39.20%,20.09%~23.57%,81.13%~95.02%and 30.33%~35.92%,respectively.No discrete data was found.Conclusion:The research of the UPLC fingerprint and the key quality attributes transmission law of Wuwei Xiaodu Yin has reference value for its development and application.

关 键 词：经典名方 五味消毒饮 物质基准 指纹图谱 量值传递 

分 类 号：R927[医药卫生—药学]