黄连根系以生理而非形态改变响应低磷胁迫  

Roots of Huanglian responded to low phosphorus stress by physiological rather than morphological changes

作　　者：李懿柔 柯汶佳 周涛 马云桐[1] LI Yi-rou;KE Wen-jia;ZHOU Tao;MA Yun-tong(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Key Laboratory of Southwestern Chinese Medicine Resources in Southwest China,Chengdu 611137,Sichuan)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中药与临床》2024年第2期20-23,共4页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:探究黄连根系响应低磷胁迫的策略,为理解栽培黄连连作障碍机制奠定基础。方法:以月为单位连续采集一周年2年生黄连样本,测定其根茎生物碱和氮(N)、磷(P)和钾(K)三种元素含量;测定水培试验黄连的生物量、根系形态、根系有机酸分泌量、营养元素含量等。结果:黄连根茎生物碱含量与磷含量成线性正相关,而与氮和钾含量无相关性。水培实验中,黄连地下部生物量在低磷和正常磷水平下无显著差异。总根长、根表面积、根体积、平均直径在两个磷浓度处理下均无差异。低磷处理中根系苹果酸和草酸分泌速率显著高于正常磷处理,以苹果酸尤为突出。根系磷含量低磷处理中显著低于高磷处理,而锌含量呈相反规律。叶片中磷、钾、铁、猛、铜含量在两个磷处理中并无明显差异。结论:黄连根系主要通过生理改变响应低磷胁迫,锌可能是影响黄连根系有机酸代谢的重要元素。Objective:To explore the Huanglian root system response strategy to low phosphorus(P)stress,and provide a basis for understanding the mechanism of successive cropping obstacle.Method:Rhizome alkaloid and nitrogen(N),P and potassium(K)contents of biennial Huanglian collected monthly for one year were detected.Biomass,root morphology,organic acid secretion and nutrient element content of Huanglian in hydroponics were determined.Result:The alkaloid content of Huanglian rhizome was linearly positively correlated with P content,but not with N and K content.In the hydroponic experiments,there was no significant difference between the biomass of Huanglian underground at low and normal P levels.The total root length,root surface area,root volume and mean diameter had no difference under two P concentration treatments.The rate of root malic acid and oxalic acid secretion in the low phosphorus treatment was significantly higher than in the normal P treatment,especially malic acid.Root P content in the low P treatment was significantly lower than in the high P treatment,while root zinc content showed the opposite pattern.There was no significant difference in leaf P,K,Fe,Mg and Cu contents between the two P treatments.Conclusion:The roots of Huanglian mainly respond to low P stress through physiological changes.Zn may be an important element affecting the organic acid metabolism of Huanglian roots.

关 键 词：黄连 根系 磷 根系形态 根系生理 

分 类 号：R282[医药卫生—中药学]