有机碳源对异养硝化-好氧反硝化生物脱氮的影响及其优化  

Effect of organic carbon on the biological nitrogen removal through heterotrophic nitrification-aerobic denitrification and its optimization

作　　者：谭丰佚 刘新颖[1] 党岩 李继云[2] 徐康宁[1] TAN Fengyi;LIU Xinying;DANG Yan;LI Jiyun;XU Kangning(Beijing Key Laboratory of Water Pollution Source Control Technology,College of Environmental Science and Engineering,Beijing Forestry University,Beijing 100083,China;School of Environment,Tsinghua University,Beijing 100084,China)

机构地区：[1]北京林业大学环境科学与工程学院,北京市水体污染源控制技术重点实验室,北京100083 [2]清华大学环境学院,北京100084

出　　处：《环境工程学报》2024年第4期1183-1191,共9页Chinese Journal of Environmental Engineering

基　　金：国家自然科学青年基金资助项目(52300075)。

摘　　要：异养硝化-好氧反硝化(HN-AD)是一种新型的高氨氮有机废水生物脱氮技术,目前在纯菌系统中的研究较多而更适合真实应用场景的混菌系统相关研究则较为缺乏,限制了技术的推广应用。本研究首先开发了具有HN-AD功能的活性污泥驯化方法并进行了验证,然后研究了碳源种类和C/N比的影响并进行了优化。结果表明,HN-AD污泥被成功驯化,自养硝化菌的丰度占比较低(<1%),在最佳脱氮效果运行条件下,Thauera逐渐富集并成为HN-AD系统的优势功能菌,其丰度占比高达69.7%。有机碳源对HN-AD脱氮系统具有重要影响,采用混合碳源(柠檬酸钠、乙酸钠、丁二酸钠,溶液中三者的COD比=1:1:1)并在C/N=20时实现了95%的氨氮去除率和89.6%的总氮去除效率,同时COD去除率高达92%,可实现高效脱氮和有机物去除。Heterotrophic nitrification-aerobic denitrification(HN-AD)is a novel biological nitrogen removal technology for high-ammonium organic wastewater.Currently,related studies mainly focus on the HN-AD in pure culture using single bacteria,while studies on mixed bacteria systems suitable for realistic application scenario are relatively scarce,limiting the application of this technology.This study developed and validated an activated sludge domestication method with HN-AD function.Subsequently,the impacts of carbon source types and C/N ratio were investigated and optimized.Results showed that the HN-AD sludge was successfully domesticated,with a low proportion of autotrophic nitrifying bacteria(<1%).Under the optimal condition of nitrogen removal,Thauera was gradually enriched and became the dominant functional bacterium in the HNAD system,accounting for a high relative abundance of 69.7%.Organic carbon sources had a significant impact on the HN-AD nitrogen removal system.At a C/N ratio of 20 and the mixed carbon sources(sodium citrate,sodium acetate,sodium succinate,the related COD ratio of these carbon sources was 1:1:1)in the HN-AD system,the removal efficiency of ammonium and total nitrogen could reach 95%and 89.6%,respectively,and COD removal efficiency could reach 92%,which could achieve effective total nitrogen and organic matter removal.

关 键 词：异养硝化-好氧反硝化 碳氮比 氨转化率 微生物群落 反应动力学 

分 类 号：X703[环境科学与工程—环境工程]