土库曼蝗线粒体基因组特征及其系统发育分析  

Characteristics and Phylogenetic Analysis of Mitochondrial Genome in Rambu-riella turcomana

作　　者：马钰洁 兰世强 蒲雪 季荣[1,2,3] 袁亮 MA Yujie;LAN Shiqiang;PU Xue;JI Rong;YUAN Liang(International Center for the Collaborative Management of Cross-border Pests in Central Asia,School of Life Sciences,Xinjiang Nor-mal University,Urumqi,830054;Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology,School of Life Sciences,Xinjiang Normal University,Urumqi,830054;Key Laboratory of Special Environment Biodiversity Application and Regu-lation in Xinjiang,School of Life Sciences,Xinjiang Normal University,Urumqi,830054)

机构地区：[1]新疆师范大学生命科学学院,中亚区域跨境有害生物联合控制国际联合研究中心,乌鲁木齐830054 [2]新疆师范大学生命科学学院,新疆特殊环境物种保护与调控生物学实验室,乌鲁木齐830054 [3]新疆师范大学生命科学学院,新疆特殊环境物种多样性应用与调控重点实验室,乌鲁木齐830054

出　　处：《基因组学与应用生物学》2024年第5期864-876,共13页Genomics and Applied Biology

基　　金：国家自然科学基金项目(32260130)资助。

摘　　要：土库曼蝗(Ramburiella turcomana)是新疆草原常见的危害蝗虫。本研究基于二代测序技术获得土库曼蝗线粒体全基因组序列,并对其进行注释及特征分析。采用近邻相接法(neighbor joining method,NJ)和贝叶斯法(bayesian inference meth-od,BI)构建网翅蝗科(Arcypteridae)系统发育树。结果表明,土库曼蝗线粒体基因为双链环状DNA,全长15670 bp,具有明显AT偏好(72.21%),共编码37个基因,包括13个蛋白编码基因、22个tRNA编码基因、2个rRNA编码基因,以及1个非编码控制区域(D-loop区)。土库曼蝗线粒体基因组发生了蝗亚目(Locustodea)普遍存在的DK重排(DK rearrangement)现象。基于网翅蝗科已报道物种线粒体全基因组构建的NJ树和BI树具有相同的拓扑结构,其中土库曼蝗与16种网翅蝗亚科(Arcypterinae)蝗虫聚为一支,并位于网翅蝗亚科最基部,其余6种竹蝗亚科(Ceracrinae)蝗虫聚为一支,暗示土库曼蝗是网翅蝗亚科中较古老的物种。本研究公布的土库曼蝗线粒体全基因组为土库曼蝗属(Ramburiella)中首次报道,为挖掘土库曼蝗属其他物种线粒体全基因组提供了分子生物学参考。Ramburiella turcomana is a common locust in the grasslands of Xinjiang.In this study,the mitochondrial whole genome se-quence of Ramburiella turcomana was obtained based on second generation sequencing technology,and the characteristics were annota-ted and characterized.A phylogenetic tree of Arcypteridae was constructed using neighbor joining method(NJ)and bayesian inference method(BI).The results showed that the Ramburiella turcomana mitochondrial genome was a double-stranded circular DNA,15670 bp in length,with a clear AT preference(72.21%),encoding a total of 37 genes,including 13 protein-coding genes,22 tRNA-coding genes,2 rRNA-coding genes,and 1 non-coding control region(D-loop region).The Ramburiella turcomana mitochondrial genome un-derwent the DK rearrangement prevalent in Locustodea.The NJ and BI trees constructed on the basis of mitochondrial whole genomes of reported species of Arcypteridae shared the same topology,with Ramburiella turcomana clustered with total 16 species of Arcypterinae locusts and located at the base of the Arcypterinae,and the remaining six species of Ceracrinae locusts clustered in one group,sugges-ting that Ramburiella turcomana was the older species of Arcypterinae.The whole mitochondrial genome of Ramburiella turcomana pub-lished in this study is the first report in Ramburiella and provides a molecular biological reference for the excavation of the whole mito-chondrial genome of other species of Ramburiella.

关 键 词：网翅蝗科 土库曼蝗 线粒体基因组 系统发育分析 

分 类 号：S812.6[农业科学—草业科学]