新疆家蚕抗菌肽靶向纳米药物载体的合成及其对宫颈癌HeLa细胞增殖的影响  

Synthesis of CecropinXJ Targeting Nanocarriers and Its Proliferation Effects on Cervical Cancer HeLa Cells

作　　者：常英豪 潘彦竹 王子烨 赵创 夏丽洁 CHANG Yinghao;PAN Yanzhu;WANG Ziye;ZHAO Chuang;XIA Lijie(National Demonstration Center for Experimental Biology Education,Xinjiang University,Xinjiang Key Laboratory of Biological Re-sources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi,830046)

机构地区：[1]新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,新疆大学生物学国家级实验教学示范中心,乌鲁木齐830046

出　　处：《基因组学与应用生物学》2024年第5期925-932,共8页Genomics and Applied Biology

基　　金：国家自然科学基金项目(32060229);国家级大学生创新训练计划项目(202210755068)共同资助

摘　　要：新疆家蚕抗菌肽(CecropinXJ,CEC)可抑制宫颈癌细胞生长,但缺乏靶向性,难以将药物精确地输送到肿瘤部位。纳米药物递送系统是解决这一问题的有效策略。本研究基于金属有机框架ZIF⁃8制备了一种新型叶酸官能化FA⁃ZIF⁃8@CEC纳米颗粒作为宫颈癌靶向药物递送系统。红外光谱、紫外⁃可见吸收光谱、核磁氢谱、X射线衍射(X⁃ray diffraction,XRD)、扫描电镜(scanning electron microscopy,SEM)、透射电镜(transmission electron microscopy,TEM)等表征结果证明成功制备了FA⁃ZIF⁃8@CEC纳米颗粒。MTT结果显示,与游离CEC和ZIF⁃8@CEC相比,FA⁃ZIF⁃8@CEC在HeLa细胞中表现出更好的抗宫颈癌效果。本研究结果说明叶酸官能化的FA⁃ZIF⁃8@CEC纳米药物在宫颈癌靶向治疗中具有一定的应用前景。CecropinXJ(CEC)can exhibit good inhibitory effect on cervical cancer cells,but it is difficult to precisely controlthe drug to arrive the tumor site.Nanomedicine delivery systems hold tremendous potential as an effective drug delivery system.In this study,a novel folate functionalized FA⁃ZIF⁃8@CEC nanoparticle based on a metal⁃organic framework was developed to transport CEC for cervi⁃cal cancer.The characterization results of infrared spectroscopy,ultraviolet⁃visible absorption spectroscopy,nuclear magnetic hydrogen spectroscopy,X⁃ray diffraction(XRD),scanning electron microscopy(SEM),transmission electron microscopy(TEM),and other methods showed that FA⁃ZIF⁃8@CEC nanoparticles were successfully prepared.MTT results showed that the FA⁃ZIF⁃8@CEC exhibi⁃ted higher anticancer efficiency in HeLa cells compared to free CEC and ZIF⁃8@CEC,indicating promising potential for targeted treat⁃ment of cervical cancer.The results show that folate⁃functionalized FA⁃ZIF⁃8@CEC nanoparticles have broad application prospects in targeted drug delivery for cervical cancer.

关 键 词：肿瘤靶向药物 抗菌肽 金属有机框架 叶酸 

分 类 号：R943[医药卫生—药剂学] R965[医药卫生—药学]