水稻硝酸盐信号核心转录因子OsNLP3氮高效单倍型分析  

Analysis of Nitrogen Efficient Haplotypes of Rice Nitrate Signaling Core Transcription Factor OsNLP3

作　　者：程功叶 于江辉 李玲玲 李文宇[1] 张迪 肖云华 段美娟[1] 刘次桃 CHENG Gongye;YU Jianghui;LI Lingling;LI Wenyu;ZHANG Di;XIAO Yunhua;DUAN Meijuan;LIU Citao(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128

出　　处：《杂交水稻》2024年第3期20-31,共12页Hybrid Rice

基　　金：国家自然科学基金(32272050,U22A20502);湖南省自然科学基金面上项目(2022JJ30021);湖南省科技创新项目(2023NK1010);长沙市科技计划(kq2202221)。

摘　　要：水稻NIN-like转录因子OsNLP3是硝酸盐信号的核心转录因子,可提高水稻对硝酸盐的吸收、同化、转运并最终提升产量。为鉴定OsNLP3基因的优异单倍型,在136份水稻核心种质资源群体中对OsNLP3基因的核苷酸等位变异与该群体对氯酸盐(硝酸盐毒性类似物)敏感表型进行关联分析。在OsNLP3基因中共鉴定出35个等位基因变异,其中19个等位基因变异(启动子区14个、编码区5个)与氯酸盐敏感表型显著关联。单倍型分析结果表明,13个等位基因变异(启动子区10个、编码区3个)对氯酸盐敏感表型起决定作用,进一步对上述关键等位基因变异进行单倍型分析,单倍型Hap2和Hap3对氯酸盐的敏感性极显著高于Hap1。此外,Hap2和Hap3分别主要由硝酸盐利用效率较高的籼稻和秋稻组成;Hap1主要由硝酸盐利用效率较低的粳稻组成。综上,OsNLP3基因的单倍型Hap2和Hap3是氯酸盐敏感单倍型,也是硝酸盐吸收利用的优异单倍型,可为后续开发OsNLP3基因分子标记及应用分子标记辅助育种提供参考。Rice NIN-like protein OsNLP3 is the core transcription factor of nitrate signaling,which can improve nitrate uptake,assimilation,transport and ultimately increase rice yield.In order to identify the excellent haplotypes of the OsNLP3 gene,the analysis was conducted on the association of the nucleotide allelic variations of OsNLP3 gene with the sensitive phenotype of the population to chlorate(a nitrate toxicity analog)in 136 core rice germplasm resources.A total of 35 allelic variations were identified in OsNLP3,of which 19 allelic variations(14 in the promoter region and five in the encoding region)were significantly associated with the chlorate-sensitive phenotype.Haplotype analysis showed that 13 allelic variations(10 in the promoter region and three in the encoding region)played a decisive role in the sensitive phenotype of chlorate.Further haplotype analysis of the above key allelic variations showed that the sensitivity to chlorate of the haplotypes Hap2 and Hap3 was significantly higher than that of the haplotype Hap1.In addition,Hap2 and Hap3 were mainly composed of indica and Aus,respectively,with higher nitrate use efficiency;Hap1 was mainly composed of japonica with lower nitrate use efficiency.In summary,the haplotypes Hap2 and Hap3 of the OsNLP3 gene are the chlorate-sensitive haplotypes with superior nitrate uptake and utilization,which can provide a reference for the subsequent development of molecular markers of the OsNLP3 gene and the application of molecular marker-assisted breeding.

关 键 词：水稻 OsNLP3 等位变异 单倍型 氮高效 

分 类 号：S511.01[农业科学—作物学] S311