长粒粳稻不育系、恢复系及其配制组合的功能基因分析  

Analysis of Functional Genes in Long-grain Japonica CMS Lines,Restorer Line and Their Hybrid Combinations

作　　者：丁正权 王士磊[1] 潘月云 施扬 黄海祥[1] DING Zhengquan;WANG Shilei;PAN Yueyun;SHI Yang;HUANG Haixiang(Jiaxing Academy of Agricultural Sciences,Jiaxing,Zhejiang 314016,China)

机构地区：[1]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《杂交水稻》2024年第3期39-47,共9页Hybrid Rice

基　　金：嘉兴市科技计划项目(2020AZ10003,2021AZ10012,2023 AZ11007);嘉兴市先锋型创新团队项目(20210801)。

摘　　要：长粒粳稻不育系嘉禾212A、嘉禾549A和恢复系NP001配制的杂交组合具有适应性广、产量高、米质优、抗性好等优点。为分析长粒粳稻不育系和恢复系品种特性及其配制组合的杂种优势,并挖掘优异的功能基因,利用SLYm1R高密度水稻全基因组SNP芯片,检测并分析了嘉禾212A、嘉禾549A、NP001、中禾优1号(嘉禾212A/NP001)和中禾优5号(嘉禾549A/NP001)中可能相关的主要功能基因。结果表明,不育系嘉禾212A和嘉禾549A基因组的粳稻区段数比例超过85.00%,属于粳稻类型;恢复系NP001基因组的籼稻、粳稻区段数分别占比66.96%、19.58%,属于籼粳偏籼类型。GS3和GW7/GL7基因调控嘉禾212A和嘉禾549A长粒型性状,ALK^(c)等位基因和Wx^(b)单倍型使不育系和恢复系及其配制组合具有较高的糊化温度和较低的直链淀粉含量。Pizt、Pid3、Pi-d2/Pid2、Pii/HIT7/pi5-1、Pi-ta等基因提高不育系和恢复系的稻瘟病抗性,而Xa26/Xa3基因增强其白叶枯病抗性。不育系和恢复系中分别含有广亲和SaF和S5-5/ORF5n基因。此外,中禾优1号和中禾优5号及其亲本携带大量优势等位基因,且不育系与恢复系在株型构建、开花时间、增加籽粒产量及提高稻瘟病抗性等多个性状实现功能基因互补。研究结果为后续亲本育种改良与籼粳亚种间杂交组合配组利用提供了科学依据。The hybrid combinations derived from the long-grain japonica rice CMS lines Jiahe 212A and Jiahe 549A and the restorer line NP001 had the advantages of wide adaptability,high yield,excellent grain quality and good resistance.To analyze the characteristics of long-grain japonica CMS lines and restorer lines and the heterosis of their hybrid combinations,and to explore their excellent functional genes,the SLYm1R high-density rice whole genome SNP chip was used to detect and analyze the possibly related major functional genes in Jiahe 212A,Jiahe 549A,NP001,Zhongheyou 1(Jiahe 212A/NP001)and Zhongheyou 5(Jiahe 549A/NP001).The results showed that the proportion of japonica rice segments in the genome of the CMS lines Jiahe 212A and Jiahe 549A was more than 85.00%,indicating that they belong to the japonica type.The number of indica rice and japonica rice segments in the genome of the restorer line NP001 accounted for 66.96%and 19.58%,respectively,indicating that it belongs to the indicaclinous type.GS3 and GW7/GL7 genes regulated the long-grain traits of Jiahe 212A and Jiahe 549A,and the ALK^(c) allele and Wx^(b) haplotype made the sterile lines and the restorer line and their hybrid combinations have higher gelatinization temperature and lower amylose content.The genes Pizt,Pid3,Pi-d2/Pid2,Pii/HIT7/pi5-1 and Pi-ta increased blast resistance of the sterile lines and the restorer line,while the genes Xa26/Xa3 increased the resistance of bacterial leaf blight.The wide compatibility genes SaF and S5-5/ORF5n were found in the sterile lines and the restorer line,respectively.In addition,Zhongheyou 1 and Zhongheyou 5 and their parents carried a large number of superior alleles,and functional gene complementarity was achieved between the sterile line and the restorer line in plant type construction,flowering time,increased grain yield and increased blast resistance.The results provided a scientific basis for further improvement of parental breeding and utilization of intersubspecific hybrid combinations between indica and jap

关 键 词：长粒粳稻 不育系 恢复系 功能基因 基因芯片 

分 类 号：S511.01[农业科学—作物学] S311