棒柄杯伞的化学成分及其抗肿瘤活性研究  

Chemical constituents from Clitocybe clavipes and their antitumor activities

作　　者：雷启军 吕杰[1] 马国需 孙照翠[3] 许旭东 魏华[4] 石磊岭[2] 逯永满 LEI Qi-jun;LYU Jie;MA Guo-xu;SUN Zhao-cui;XU Xu-dong;WEI Hua;SHI Lei-ling;LU Yong-man(Xinjiang University,Urumqi 830046,China;Xinjiang Institute of Traditional Chinese Medicine and Ethnic Medicine,Urumqi 830002,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China;Jishou University,Jishou 416000,China)

机构地区：[1]新疆大学,新疆乌鲁木齐830046 [2]新疆维吾尔自治区中药民族药研究所,新疆乌鲁木齐830002 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193 [4]吉首大学,湖南吉首416000

出　　处：《中国中药杂志》2024年第11期2947-2952,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(8216130391,81960774)。

摘　　要：对棒柄杯伞进行化学成分研究,采用硅胶、ODS、Sephadex LH-20柱色谱和半制备液相等多种色谱学技术对棒柄杯伞乙醇提取物进行分离纯化,通过^(1)H-NMR、^(13)C-NMR以及质谱等多种波谱学方法共鉴定出6个化合物,分别为clavilactone L(1)、clavilactone A (2)、clavilactone B (3)、clavilactone E (4)、clavilactone H (5)和clavilactone I (6)。其中,化合物1是1个新的苯并十元碳环混源萜。通过MTT法测定了化合物1~6的抗肿瘤活性,结果表明化合物1~6对人胃癌细胞(MGC-803)、人非小细胞肺癌细胞(A549)、宫颈癌细胞(HeLa)具有不同程度的增殖抑制作用。其中化合物1对人胃癌细胞MGC-803具有较显著的抑制活性,IC_(50)为11.76μmol·L^(-1)。This paper aimed to study the chemical constituents from Clitocybe clavipes.Silica gel,ODS,Sephadex LH-20,and semi-preparative HPLC were employed to separate the ethanol extract of C.clavipes.Six compounds were identified by 1H-NMR,13CNMR,and ESI-MS as clavilactone L(1),clavilactone A(2),clavilactone B(3),clavilactone E(4),clavilactone H(5),and clavilactone I(6).Among them,compound 1 was a new meroterpenoid with a 10-membered carbocycle connected to a hydroquinone.The antitumor activities of compounds 1-6 were determined by the methyl thiazolyl tetrazolium(MTT)assay.The results showed that compounds 1-6 exerted inhibitory effects on the proliferation of human gastric cancer cells(MGC-803),human non-small cell lung cancer cells(A549),and cervical cancer cells(HeLa).Compound 1 exhibited significant inhibitory activity against MGC-803 cells,with the half maximal inhibitory concentration(IC_(50))of 11.76μmol·L^(-1).

关 键 词：棒柄杯伞 化学成分 混源萜 clavilactone L 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]