UPLC-PDA波长切换法同时测定红参和黑参中麦芽酚及17种皂苷的含量  

Simultaneous determination of maltol and seventeen saponins in red and black ginseng by UPLC-PDA wavelength switching method

作　　者：吴雨桐 许煊炜[2] 王艳红[2] 赵丹[2] 刘刚 刘育辰 WU Yu-tong;XU Xuan-wei;WANG Yan-hong;ZHAO Dan;LIU Gang;LIU Yu-chen(School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;Ginseng and Antler Products Testing Center,Ministry of Agriculture,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]吉林农业大学农业农村部参茸产品质量检验测试中心,吉林长春130118

出　　处：《中国中药杂志》2024年第11期2965-2972,共8页China Journal of Chinese Materia Medica

基　　金：吉林省科技发展计划重点研发项目(20210204021YY);吉林省科技发展计划项目(20210506009ZP)。

摘　　要：建立了UPLC-PDA波长切换法同时测定红参和黑参中麦芽酚及17种皂苷含量的方法,并比较红参和黑参2种不同人参加工品之间的质量差异。采用Waters HSS T3色谱柱(2.1 mm×100 mm,1.8μm),以乙腈(A)-0.1%磷酸水(B)为流动相进行梯度洗脱,流速0.3 m L·min^(-1),柱温30℃,进样量2μL,波长切换方式0~11 min为273 nm,11~60 min为203 nm。对多批次红参和黑参样品含量测定结果进行聚类分析(HCA)和主成分分析(PCA),评价其质量差异性。结果显示,18种目标成分在一定质量浓度范围内均呈良好的线性关系,相关系数(r)均大于0.999 1;精密度、重复性和稳定性的相对标准偏差(RSD)均小于5.0%;平均加样回收率为95.93%~104.2%,RSD为1.8%~4.2%。定量测定结果显示红参和黑参样品间存在一定差异,并且HCA和PCA这2种分析方式均可直观区分红参和黑参2类人参加工品。该方法准确、可靠、重复性好,可用于红参和黑参中麦芽酚及17种皂苷成分的含量测定,同时为红参和黑参的质量评价和综合利用提供基础资料。This study developed a UPLC-PDA wavelength switching method to simultaneously determine the content of maltol and seventeen saponins in red and black ginseng and compared the quality differences of two different processed products of red and black ginseng.A Waters HSS T3 column(2.1 mm×100 mm,1.8μm)at 30℃was adopted,with the mobile phase of acetonitrile(A)and water containing 0.1%phosphoric acid(B)under gradient elution,the flow rate of 0.3 mL·min^(-1),and the injection volume of 2μL.The wavelength switching was set at 273 nm within 0-11 min and 203 nm within 11-60 min.The content results of multiple batches of red and black ginseng samples were analyzed by the hierarchical cluster analysis(HCA)and principal component analysis(PCA)to evaluate the quality difference.The results showed that the 18 constituents exhibited good linear relationships within certain concentration ranges,with the correlation coefficients(r)greater than 0.9991.The relative standard deviations(RSDs)of precision,repeatability,and stability were all less than 5.0%.The average recoveries ranged from 95.93%to 104.2%,with an RSD of 1.8%-4.2%.The content determination results showed that the quality of red and black ginseng samples was different,and the two types of processed products were intuitively distinguished by HCA and PCA.The method is accurate,reliable,and reproducible.It can be used to determine the content of maltol and seventeen saponins in red and black ginseng and provide basic information for the quality evaluation and comprehensive utilization of red and black ginseng.

关 键 词：红参 黑参 超高效液相色谱-二极管阵列检测器 波长切换法 麦芽酚 皂苷 

分 类 号：R284.1[医药卫生—中药学]