大豆花叶病毒抗性基因及分子标记研究进展  

Progress on Studies of Resistance Genes and Molecular Markers of Soybean Mosaic Virus

作　　者：王大刚[1] 黄志平[1] 杨勇[1] 李杰坤[1] 吴倩[1] WANG Dagang;HUANG Zhiping;YANG Yong;LI Jiekun;WU Qian(Crop Institute of Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province,Hefei 230031)

机构地区：[1]安徽省农业科学院作物研究所/安徽省农作物品质改良重点实验室,合肥230031

出　　处：《植物遗传资源学报》2024年第6期882-897,共16页Journal of Plant Genetic Resources

基　　金：国家重点研发计划项目(2023YFD1401000);安徽省农业科学院青年英才项目(QNYC-201909);国家大豆产业技术体系项目(CARS-04-PS07)。

摘　　要：大豆花叶病毒(SMV,soybean mosaic virus)病是世界大豆主产区广泛存在且普遍发生的主要病害之一,对大豆的产量和品质均可造成严重危害。本文综合分析了近年来通过遗传定位发现的大豆花叶病毒抗性基因及其紧密连锁的分子标记,探讨了分子标记在提高大豆抗病育种中的应用,总结了R_(SC3(w))、R_(SC14-r)和R_(SC18)等抗大豆花叶病毒基因的物理位置及其候选基因。基于候选基因测序、实时荧光定量PCR、病毒介导基因沉默(VIGS,virus induced gene silencing)和基因编辑CRISPR/Cas9等技术梳理出GsCAD1、GmCAL和GmMLRK1等一系列直接或间接参与大豆花叶病毒抗性的相关基因,为大豆抗大豆花叶病毒基因调控网络的完善奠定了基础。本综述总结分析了大豆与大豆花叶病毒的相互作用机制,聚焦分析大豆抗性基因Rsv3等对大豆花叶病毒抗病机制研究进展,并对抗大豆花叶病毒育种的研究方向提出了展望,以期为大豆抗性基因分子标记的应用和分子调控机制的研究提供参考。Soybean mosaic virus(SMV)occurs in nearly all soybean-growing areas worldwide and it causes severe yield loss and seed quality reduction in soybean.This review summarizes the recent progress in identification of SMV resistance genes and linked molecular markers,and discusses pyramiding of multiple molecular markers to facilitate breeding of soybean varieties resistant to SMV,and analyzes physical position of SMV resistance genes,for example R_(SC3(w))、R_(SC14-r) and R_(SC18) etc.Many candidate genes(such as GsCAD1,GmCAL and GmMLRK1)were proposed based on sequence variation,quantitative real-time polymerase chain reaction(qRT-PCR),virus induced gene silencing(VIGS)and CRISPR(clustered regularly interspersed short palindromic repeats)/Cas9.Collectively,this study highlights the molecular mechanism of SMV-soybean interactions,e.g.the progress of the Rsv3 gene conferring SMV resistance,and provides perspectives for future studies via applying the markers or mechanism for SMV resistance breeding.

关 键 词：大豆 病毒 抗性 分子标记 基因 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]