大豆铝激活苹果酸转运家族基因GmALMT8的鉴定与功能验证  

Identification and Functional Analysis of Aluminum-Activated Malate Transporter Family Gene GmALMT8 in Soybean

作　　者：刘慧 许文静[1,2] 张红梅 刘晓庆[1] 张威[1] 王琼 朱月林[2] 陈华涛[1] LIU Hui;XU Wenjing;ZHANG Hongmei;LIU Xiaoqing;ZHANG Wei;WANG Qiong;ZHU Yuein;CHEN Huatao(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014;College of Horticulture,Nanjing Agricultural University,Nanjing 210095)

机构地区：[1]江苏省农业科学院经济作物研究所,南京210014 [2]南京农业大学园艺学院,南京210095

出　　处：《植物遗传资源学报》2024年第6期1027-1034,共8页Journal of Plant Genetic Resources

基　　金：江苏省农业科技自主创新资金项目(CX(22)5002)。

摘　　要：鲜食风味是影响菜用大豆食味品质的关键因素,其形成与有机酸有着密切的关联,研究有机酸合成机制对于菜用大豆的品质改良具有重要的实际意义。本研究利用大豆毛状根系统,探究与苹果酸含量显著相关的候选基因GmALMT8、GmIF7GT5和GmAP在调控苹果酸含量方面的功能,结果表明:在GmALMT8-OE毛状根中,GmALMT8基因表达量与苹果酸的含量均显著高于空载对照毛状根,而GmIF7GT5和GmAP毛状根中苹果酸的含量无显著变化。鉴于已经报道的ALMT家族基因的苹果酸转运功能,推测大豆中GmALMT8基因可能具有相似的功能,在调控苹果酸含量方面发挥重要作用。为验证GmALMT8-OE毛状根中苹果酸含量的变化是否由GmALMT8基因表达的改变所引起,本研究采用蘸花法在拟南芥中过表达GmALMT8基因。与阳性毛状根中苹果酸测定的结果相类似,过表达GmALMT8显著提高了T2代转基因拟南芥株系种子中的苹果酸含量,进一步证明GmALMT8的稳定表达能够提高苹果酸含量,明确GmALMT8基因在大豆中具有调控苹果酸含量的生物学功能,丰富了大豆有机酸的理论研究,对菜用大豆优质育种具有参考价值。Fresh flavor is the key factor of the taste quality of vegetable soybean,and its formation is closely related to the organic acids.It is of great practical significance to analyze the synthesis mechanism of organic acids in quality improvement of vegetable soybean.In this study,the soybean hairy root system was used to explore the functions of candidate genes GmALMT8,GmIF7GT5 and GmAP,which are significantly related to malic acid content,in regulating malic acid content.The transcripts of GmALMT8 gene and the content of malic acid in GmALMT8-OE hairy roots were significantly higher than those in control hairy roots without inserts,while the content of malic acid in GmIF7GT5 and GmAP transformed hairy roots had no significant change.In view of the reported malic acid transport function of ALMT family genes,it is speculated that GmALMT8 gene in soybean may have a similar function and play an important role in regulating malic acid content.In order to verify whether the change of malic acid content in GmALMT8-OE hairy roots is caused by the change of GmALMT8 gene expression,this study overexpressed GmALMT8 gene in Arabidopsis thaliana by dipping flower method.Similar to the results of malic acid determination in positive hairy roots,overexpression of GmALMT8 significantly increased the malic acid content in seeds of T2 transgenic Arabidopsis thaliana.These results approved that soybean GmALMT8 gene had biological function in regulating malic acid content,which enriched the theoretical research of soybean organic acid and provided reference value for high-quality breeding of vegetable soybean.

关 键 词：菜用大豆 苹果酸 候选基因 功能分析 

分 类 号：S643.7[农业科学—蔬菜学]