番茄种质资源萌发期抗旱性综合评价及筛选  

Comprehensive Evaluation and Screening of Drought Resistance of Tomato Germplasm Resources during Germination Period

作　　者：马越 李玉姗 王帆 许红军 宋羽[1,3] 蒋程瑶 MA Yue;LI Yushan;WANG Fan;XU Hongjun;SONG Yu;JIANG Chengyao(Institute of Crop Variety Resources,Xinjiang Academy of Agricultural Sciences,Urumqi 830091;College of Horticulture,Xinjiang Agricultural University,Urumqi 830052;National Central Asian Characteristic Crop Germplasm Resources Medium-term Gene Bank,Urumqi 830091;College of Horticulture,Sichuan Agricultural University,Chengdu 611130)

机构地区：[1]新疆农业科学院农作物品种资源研究所,乌鲁木齐830091 [2]新疆农业大学园艺学院,乌鲁木齐830052 [3]国家中亚特色作物种质资源中期库,乌鲁木齐830091 [4]四川农业大学园艺学院,成都611130

出　　处：《植物遗传资源学报》2024年第7期1056-1069,共14页Journal of Plant Genetic Resources

基　　金：新疆维吾尔自治区重大科技专项(2022A02005-2);新疆蔬菜产业技术体系(XJARS-07);四川省自然科学基金项目(2022NSFSC1759)。

摘　　要：番茄作为重要的蔬菜作物之一,鉴定番茄种质资源的抗旱性及筛选抗旱番茄种质资源对番茄育种具有重要意义。本研究利用12.5%PEG-6000溶液对92份番茄种质资源进行干旱胁迫处理,测定发芽势、发芽率、发芽指数、活力指数、根长、芽长等6个抗旱性相关指标,运用相关性分析、主成分分析、隶属函数法和聚类分析进行番茄萌发期抗旱性评价及筛选。结果表明,干旱胁迫下6个指标均显著降低,其中活力指数和发芽势降低最大。通过主成分分析将6个指标转化为2个新指标,累计贡献率达86.657%。基于相关性和主成分分析,筛选出发芽势、发芽率、发芽指数、活力指数作为萌发期抗旱性鉴定的主要指标,利用隶属函数法结合聚类分析将番茄种质资源分为4类,其中抗旱型19份、较抗旱型41份、较敏感型18份、敏感型14份;通过增大、减小PEG-6000溶液浓度,进一步筛选出抗旱性强的密植红番茄和锦农矮秧两个品种,敏感性强的北京早红和大红袍两个品种。本研究结果可为番茄种质资源的抗旱性研究提供理论基础。Tomato,being one of the important vegetable crops,holds great significance in evaluating the drought resistance of tomato germplasm resources and selecting drought-resistant varieties for tomato breeding.In this study,we subjected 92 tomato germplasm resources to drought stress treatment using a 12.5%PEG-6000solution.Six drought-related indexes,including germination potential,germination rate,germination index,vigor index,root length and shoot length,were measured,and the drought resistance of tomato at germination stage was evaluated and screened by correlation analysis,principal component analysis,membership function method and cluster analysis.The results showed a significant reduction in all six indicators under drought stress,with the vigor index and germination potential being the most affected.Through principal component analysis,we transformed the six indicators into two new indicators,which contributed to a cumulative contribution rate of 86.657%.Based on correlation and principal component analysis,we identified germination potential,germination rate,germination index,and vigor index as the main indicators for evaluating drought resistance during the germination period.By using the subordination function method combined with cluster analysis,we classified the tomato germplasm resources into four types,including 19 drought-resistant types,41 relatively drought-resistant types,18 relatively sensitive types,and 14 sensitive types.By adjusting the concentration of PEG-6000 solution,further screening of the drought-resistant varieties Mizhihong Tomato and Jinnongaiyang,as well as the sensitive varieties Beijingzaohong and Dahongpao can be conducted.These findings provide a theoretical basis for the study of drought resistance in tomato germplasm resources.

关 键 词：番茄 萌发期 抗旱性 综合评价 筛选 

分 类 号：S641.2[农业科学—蔬菜学]