两个柠檬品种叶片离区响应乙烯利处理的转录组分析  

Transcriptome Analysis of Leaf Abscission Zones Post Ethephon Treatment in Two Lemon Varieties

作　　者：董美超[1] 尹拓 周东果[1] 张汉尧 杨帆[1] 王绍华[1] 龙春瑞[1] 付小猛[1] 刘红明[1] 郭莉娜 李丹萍 高俊燕[1] DONG Meichao;YIN Tuo;ZHOU Dongguo;ZHANG Hanyao;YANG Fan;WANG Shaohua;LONG Chunrui;FU Xiaomeng;LIU Hongming;GUO Lina;LI Danping;GAO Junyan(Institute of Tropical Subtropical Economic Crops,Yunnan Academy of Agricultural Sciences,Baoshan 678000;College of Forestry,Southwest Forestry University,Kunming 650224)

机构地区：[1]云南省农业科学院热带亚热带经济作物研究所,保山678000 [2]西南林业大学林学院,昆明650224

出　　处：《植物遗传资源学报》2024年第7期1199-1210,共12页Journal of Plant Genetic Resources

基　　金：国家自然科学基金(31960574);英才兴边计划(2022RC001);创新引导与科技型企业培育计划(202304BI090025)。

摘　　要：艾伦尤力克是尤力克柠檬的一个芽变品种,结果性状优良,但冬季落叶严重,影响翌年产量,且其落叶的响应机制目前尚不清楚。为探究柠檬叶片脱落的分子调控机制,以2个冬季落叶程度不同的柠檬品种艾伦尤力克和云柠1号为材料,分别于落叶前期(E24)、落叶中期(E48)和落叶后期(E72)采集叶柄离区,通过转录组测序比较2个品种间的差异表达基因。分析表明,落叶前期、落叶中期和落叶后期分别获得1400个、2466个和935个差异表达基因,其中落叶中期的差异基因数量最多。GO分析表明血红素结合、四吡咯结合、氧化还原酶活性、铁离子结合、转录调节活性以及对氧化应激的反应、细胞葡聚糖代谢过程、葡聚糖代谢过程、细胞外围、细胞壁等相关基因在这3个时期品种间均表现出显著差异。KEGG分析表明,落叶中期富集的差异基因数及相关代谢途径最多,主要集中在植物激素信号转导、苯丙烷生物合成、植物病原体相互作用和MAPK信号通路-植物等途径,通过对以上4个途径的差异表达显著的基因进行分析,最后筛选得到木葡聚糖内转葡糖基酶/水解酶蛋白、吲哚乙酸诱导蛋白、吲哚-3-乙酸-氨基合成酶、过氧化物酶、β-葡萄糖苷酶、发病相关基因转录激活因子AP2和发病机制相关蛋白等13个基因,这些基因可能与柠檬叶片脱落的调控有关。qRT-PCR验证这些基因的表达与转录组数据相一致。以上结论丰富了柠檬叶片脱落相关研究,为柠檬落叶候选基因筛选、落叶途径解析提供可靠数据。'Allen Eureka'is a progeny variety of the variety‘Eureka lemon'due to bud mutation.This variety showed excellent performance at fruiting traits,while it was susceptible to winter defoliation,eventually resulting in severe leaf shedding and decreasing the yield production in the coming year.The mechanism of leaf shedding remains unknown.Two lemon cultivars(‘Allen Eureka'and‘Yunning No.1')with degrees of winter defoliation were used to investigate the molecular regulatory mechanisms of leaf abscission.The petiole abscission zones were collected at three stages,namely,the pre-defoliation stage(E24),mid-defoliation stage(E48),and post-defoliation stage(E72),and subjected for transcriptome sequencing.The differentially expressed genes(DEGs)between two lemon varieties were identified,revealing a total of 14002466,and 935DEGs in pre-defoliation stage,mid-defoliation stage,and post-defoliation stage,respectively.The largest number of DEGs was found in mid-defoliation stage.GO enrichment analysis revealed that these DEGs were mainly enriched in the processes of heme binding,tetrapyrrole binding,oxidoreductase activity,iron ion binding,transcription regulator activity and response to oxidative stress,cellular glucan metabolic process,glucan metabolic process,cell periphery,cell wall in the defoliation stages.KEGG analysis showed that the DEGs were concentrated in mid-defoliation stage and involved in plant hormone signal transduction,phenylpropanoid biosynthesis,plant-pathogen interaction,and MAPK signaling pathway.By analyzing the genes with significant differential expression of the four pathways,13 genes including xyloglucan endotransglucosylase/hydrolase protein,indoleacetic acid-induced protein,indole-3-acetic acid-amido synthetase,peroxidase,β-glucosidase,pathogenesis-related genes transcriptional activator AP2 and pathogenesis-related protein were selected.They might be associated with the regulation of lemon leaf abscission.qRT-PCR verified that the expression of these genes was consistent with the transcrip

关 键 词：柠檬 落叶 转录组 差异表达基因 

分 类 号：S666.5[农业科学—果树学]