海洋真菌Aspergillus sp.LW152抗细菌活性代谢产物研究  

Study on antibacterial secondary metabolites from the marine-derived fungus Aspergillus sp.LW152

作　　者：刘仟茜 王茹 蔡磊[2,3] 殷军 刘玲[1,2,3] Liu Qianqian;Wang Ru;Cai Lei;Yin Jun;Liu Ling(School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shengyang 110016;State Key Laboratory of Mycology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101;University of Chinese Academy of Sciences,Beijing 100049)

机构地区：[1]沈阳药科大学中药学院,沈阳110016 [2]中国科学院微生物研究所真菌学国家重点实验室,北京100101 [3]中国科学院大学,北京100049

出　　处：《中国抗生素杂志》2024年第6期627-633,共7页Chinese Journal of Antibiotics

基　　金：国家自然科学基金项目(No.32022002)。

摘　　要：目的对海洋真菌Aspergillus sp.LW152发酵提取物的抗细菌成分进行研究。方法利用硅胶柱层析、葡聚糖凝胶Sephadex LH-20柱层析、半制备高效液相色谱等方法,对该菌株发酵提取物进行分离纯化;化合物结构通过核磁共振波谱(NMR)、质谱(MS)等数据并与文献数据对比进行确定;采用微量肉汤稀释法对化合物抗菌活性进行体外评价。结果从真菌LW152发酵的乙酸乙酯提取物中分离得到了7个化合物,其结构分别鉴定为7-脱氧-7,14-二脱氢水杨酸(1)、7-脱氧-7,8-二脱氢水杨酸(2)、pseudaboydin B(3)、(Z)-5-(羟甲基)-2-(6'-甲基庚-2'-烯-2'-基)苯酚(4)、aspergillusene A(5)、2,4-二羟基-6-甲基苯甲酸甲酯(6)和1-亚油酸单甘油酯(7)。结论海洋真菌Aspergillussp.LW152能产生具有抗细菌活性的次级代谢产物,其中化合物4和5对6种致病细菌的生长均具有一定的抑制作用,化合物7对青枯雷尔菌的生长具有抑制作用。Objective This research investigated the antibacterial secondary metabolites from the fermentation extracts of the marine fungus Aspergillus sp.LW152.Methods Silica gel column chromatography,Sephadex LH-20 gel column chromatography and semi-preparative high-performance liquid chromatography(RP HPLC)were used to isolate and purify the secondary metabolites from the fermented extracts of the fungus Aspergillus sp.LW152.The structures of these secondary metabolites were determined by analysis of their NMR and MS spectroscopic data and by comparison with those in the literatures.The antibacterial activity was evaluated by the 96-well plate broth microdilution method.Results Seven compounds were isolated from fermented ethyl acetate extracts of the fungus LW152 and identified as 7-deoxy-7,14-didehydrosydonic acid(1),7-deoxy-7,8-didehydrosydonic acid(2),pseudaboydin B(3),(Z)-5-(hydroxymethyl)-2-(6'-methylhept-2'-en-2'-yl)phenol(4),aspergillusene A(5),methyl orsellinate(6)and 1-monolinolein(7).Conclusion The marine-derived fungus Aspergillus sp.LW152 could produce secondary metabolites with antibacterial activities.Compounds 4 and 5 showed antibacterial activities against six kinds of pathogenic bacteria.While compound 7 showed antibacterial activity against Ralstonia solanacearum.

关 键 词：海洋真菌 曲霉属 分离纯化 结构鉴定 次级代谢产物 抗菌活性 

分 类 号：R978.1[医药卫生—药品]