辣椒细胞核雄性不育材料GMS702AB的鉴定  

Identification of Nuclear Male Sterile Material GMS702AB in Pepper

作　　者：汪胜 贾利[2] 唐菁 李浩宇 宋婷婷 袁娟伟 严从生[2] 方凌[2] 张其安[2] 孙玉军 江海坤[2] 孙学良 张涛[2] WANG Sheng;JIA Li;TANG Jing;LI Haoyu;SONG Tingting;YUAN Juanwei;YAN Congsheng;FANG Ling;ZHANG Qian;SUN Yujun;JIANG Haikun;SUN Xueliang;ZHANG Tao(College of Agriculture,Anhui Science and Technology University,Fengyang 233100;Institute of Horticulture,Anhui Academy of Agricultural Sciences/Anhui Provincial Key Laboratory for Germplasm Resources Creation&High-Efficiency Cultivation of Horticultural Crops/Key Laboratory of Germplasm Creation and Utilization of Horticultural Crops,Ministry of Agriculture and Rural Affairs(Co-comstruction by Ministry and Province),Hefei 230031;College of Horticulture,Anhui Agricultural University,Hefei 230036)

机构地区：[1]安徽科技学院农学院,凤阳233100 [2]安徽省农业科学院园艺研究所/园艺作物种质资源创制及高效栽培安徽省重点实验室/农业农村部园艺作物种质创制与利用重点实验室(部省共建),合肥230031 [3]安徽农业大学园艺学院,合肥230036

出　　处：《植物遗传资源学报》2024年第4期612-621,共10页Journal of Plant Genetic Resources

基　　金：财政部和农业农村部国家现代农业产业技术体系(CARS-23-G40,CARS-23-G49);国家重点研发计划(2023YFD1201500);安徽省农业科学院青年英才经费(QNYC-202121);安徽省科技重大专项(202203a06020030);安徽省蔬菜产业技术体系专项(皖农科函[2021]711号);安徽省辣椒良种联合攻关项目。

摘　　要：本研究发现1个具有雄性不育与单性结实特征的细胞核雄性不育系辣椒材料,并对该材料的农艺性状、单性结实坐果率、不同发育时期的不育系单性结实与可育系单性结实内源激素进行测定;利用田间鉴定和显微镜镜检,分析了F2群体的遗传分离情况,并利用辣椒细胞核雄性不育基因ms-3、msw、ms、msms、msc-1开发的分子标记,分析了群体的育性分离比。结果表明:不育系单性结实果实纵横径较大,可育系与不育系单性结实坐果率明显不同,可育系坐果率为22%,不育系坐果率为43%;不同时期不育系单性结实果实的赤霉素(GA4)含量显著高于可育系单性结实果实;田间鉴定和分子标记检测表明,F2群体中可育系有97株,不育系有30株,分离比为3.23∶1,符合孟德尔遗传规律,确定其育性由隐性单基因控制,将该辣椒细胞核雄性不育系命名为GMS702AB。本研究提供新的辣椒不育系,有助于辣椒育种和种子生产。This study identified a nuclear male sterile pepper line showing male sterility and parthenocarpy characteristics,and the agronomic traits and parthenocarpy fruit setting rate of the material were determined,and the parthenocarpy of the sterile line at different developmental stages and the parthenocarpy of the fertile line were determined for endogenous hormones.In addition,the genetic segregation of F2 population was analyzed by field identification and microscopic examination,and the fertility segregation ratio of the population was analyzed by using the molecular markers developed by pepper nuclear male sterile genes ms-3,msw,ms,msms and msc-1.The results showed the vertical and horizontal diameters of the parthenocarpy fruit of the sterile line were larger,and the fruit setting rate of the fertile line and the sterile line was significantly different.The fruit setting rate of the fertile line was 22%,and the fruit setting rate of the sterile line was 43%.The GA4(gibberellin)content in the parthenocarpic fruit of the sterile line in different periods was significantly higher than that of the fertile line.There are 97 fertile lines and 30 sterile lines in the F2 population,with a separation ratio of 3.23:1,which is consistent with the Mendelian inheritance law.It showsd that its fertility was controlled by a recessive single gene.We named the nuclear male sterile line of pepper as GMS702AB.This study provides a new nuclear male sterile line valuable in pepper breeding and seed production.

关 键 词：辣椒 雄性不育 分子标记 单性结实 

分 类 号：S641.3[农业科学—蔬菜学]